Exceptional value for money Rapid detection of all clinically relevant subtypes Positive copy number standard curve for quantification Highly specific detection profile High priming efficiency Broad dynamic detection range (>6 logs) Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Product Info
Document
Product Info
High Purity DNA Purification Kit
,
12 Months DNA Purification Kit
,
Nucleic Acid dna isolation kit
Product Description
Rapid Nucleic Acid Release Reagent (DNA) – Type II
High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Product parameters:
Reagent component (WLD8201-ES,2Tubes/box)
Component
Specification
Quantity
LD-1
800μL＝0.8mL
1 Tube
LD-2
200μL＝1.2mL
1 Tube
Product features and advantages:
Product features
Simplicity
The operation is simple, and the DNA template for amplification can be obtained in just one step (conventional 5min)
Security
Non-toxic environmental protection, room temperature transport and storage
Universality
The lysate can be used for isothermal nucleic acid amplification
Product application:
It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.
Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.
Sample processing type
Liquid sample
Samples of plants or tissues
Serum, blood, saliva, ascites and other samples, swab leach solution
Brain, muscle, viscera, leaves, fruit and other samples, take liquid supernatant
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications
RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Cells: ≤1 x 107 Animal tissue: 1-20 mgPlant leaves: 50-150 mg Yeast cells: 5 x 106
Yield
2-100μg
Elution volume
≥50μl
Time per run
≤15 minutes（1-24 samples）
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 20 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
R401102
R401103
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
RTL Lysis Buffer
50 ml
200 ml
RNA Binding Buffer
15 ml
75 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
