Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
DOTA-PEG5-C6-DBCO
Product Info
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Product Info
DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DOTA-PEG5-C6-DBCO is a PEG linker containing DOTA and DBCO moieties. DBCO is used in Click Chemistry reactions due to its high strain energy. The DOTA group can be ionized and is susceptible for chelating di- and trivalent cations. DOTA can also be used for imaging diagnostic techniques. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
X96 Dual Ring Magnetic Plate 96-well with Integrated Cushion Base
Product Info
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Product Info
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL (from PCR plates), larger outer magnet handles up to 2 mL deep well assays
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom, SBS/ SLAS fit on top to accept any microplate
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable differences
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
Permagen’s most universal ring magnet plate. From Low elution PCR applications up to 2 mL deep well, the X96 has you covered. No need to purchase two separate plates anymore. Smaller inner ring magnet allows for volumes as low as 5 µL (from PCR plates), larger outer magnet handles up to 2 mL deep well assays
For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017.
Principle and Interpretation
Meat extract and casein provide a source of nitrogen and amino acids and sodium chloride maintain theosmotic balance. Ox bile and brilliant green act as selective agents against non-target microorganisms. Tetrathionate is generated from the sodium thiosulfate. Iodine and calcium carbonate buffer the sulfuric acid generated from tetrathionate reduction.
Formulation
Ingredients
/liter
Meat extract
4.3g
Enzymatic digest of casein
8.6g
Sodium chloride
2.6g
Calcium carbonate
38.7g
Sodium Thiosulfate (anhydrous)
30.4g
Ox bile
4.78g
pH8.0±0.2 at 25°C
Preparation
Suspend 89.4g in 1 L of purified water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks，and then cool to below 45°C. Add a vial of novobiocin sodium salt (SR0640), a vial of iodine solution and a vial of brilliant green (SR0040) into 100 mL of base medium. Mix thoroughly.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 24 hours
Quality control strains
Approx. Inoculum(CFU)
Expected Results
Salmonella typhimurium ATCC14028
10 – 100
≥ 10 cfu on XLD
Escherichia coli ATCC25922
> 104
≤100 cfu on TSA
Enterococcus faecalis ATCC29212
> 104
<10 cfu on TSA
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
ISO 6579:2017 Microbiology of food and animal feeding stuffs – Horizontal method for the detection, enumeration and serotyping of Salmonella spp.
Document
Intended Use For the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017. Principle and Interpretation……
