Introduction

MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.

Details

Specifications

Features	Specifications
Main Functions	Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP)
Applications	NGS, DNA library
Purification technology	Magnetic beads technology
Process method	Manual (centrifugation or vacuum)
Sample type	DNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount	Appropriate
Recovery	80%
Operation time	≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.

Advantages

High recovery – up to 80%
High throughput – using magnetic beads purification technology

Kit Contents

Contents	BP-5	BP-50	BP-500
MagPure A4 XP	5 ml	50 ml	500 ml

Storage and Stability

MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.

DO NOT FREEZE.

Kit Specifications
Volume of Urine Processed	1.75 mL
Average Yield*	Up to 50 ng
Size of DNA Purified	Large (> 1 kb) and small (150-250 bp)
Time to Complete 10 Purifications	30 minutes hands-on time (plus a 1 hour incubation)

Component	Cat. 18100 (50 preps)	Cat. 48800 (50 preps)	Cat. 50100 (50 preps)
Binding Solution K	15 mL	–	–
Slurry B1	–	18 mL	18 mL
Proteinase K in Storage Buffer	2 mL	–	–
Pronase K in Storage Buffer	2 mL	–	–
Soluton WN	9 mL	–	–
Binding Buffer A	–	–	50 mL
Lysis Buffer A	–	30 mL	30 mL
Wash Solution A	–	38 mL	38 mL
Wash Solution B	30 mL	–	–
Wash Solution D	9 mL	–	–
Binding Solution K	15 mL	–	–
Elution Buffer B	15 mL	15 mL	15 mL
Micro Spin Columns	50	–	–
Mini Filter Spin Columns	–	50	50
Collection Tubes	50	50	50
Elution Tubes (1.7 mL)	100	100	100
Product Insert	1	1	1

Features	Specifications
Main Functions	Isolation total RNA from bacteria, yeast cells
Applications	RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Bacteria, yeast cells
Sample amount	Bacteria: <10^9；Yeast cells：<2 x10^7
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Contents	R418202	R418203
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
Glass Beads (0.1-0.6mm)	30 g	150 g
DNase I	600 μl	5 x 600 μl
DNase Buffer	6 ml	30 ml
Protease Dissolve Buffer	1.8 ml	15 ml
Buffer ATL	50 ml	200 ml
Buffer PHC	50 ml	200 ml
Buffer GXP2*	20 ml	100 ml
Buffer RW1	50 ml	250 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

BP MagPure A4 XP

Detail

Introduction

Details

Principle

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