MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Detail
Introduction
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Details
Specifications
Features
Specifications
Main Functions
Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP)
Applications
NGS, DNA library
Purification technology
Magnetic beads technology
Process method
Manual (centrifugation or vacuum)
Sample type
DNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount
Appropriate
Recovery
80%
Operation time
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
High recovery – up to 80%
High throughput – using magnetic beads purification technology
Kit Contents
Contents
BP-5
BP-50
BP-500
MagPure A4 XP
5 ml
50 ml
500 ml
Storage and Stability
MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
Other Products
Pilot-Scale Freeze Dryer
Product Info
Document
Product Info
Character
High flatness sample shelf:
Silicone oil thermal conductive shelf, high-precision processing method of upper and lower clamping, shelf temperature uniformity can reach ±0.5°c
Integrated cavity:
Integrated chamber, the shelf and cold trap are located in the same chamber, the steam conduction is faster and the freeze-drying effect is better
Refrigerating and heating control system:
Using thermally conductive silicone oil with a high temperature range and precise PID control algorithm, the control accuracy can reach ±0.5°C. Sample shelf temperature range: -70°C~ +80°C
Freeze-drying endpoint testing system:
The pressure comparison method is used to determine the freeze-drying end point. The freeze-drying end point test can be automatically performed after the analytical drying stage to ensure that the moisture content of the material reaches the standard requirements.
Vacuum precise control and adjustment system:
The sublimation and analytical drying processes are controlled and adjusted with high precision to make the freeze-drying process warmer and the sample sublimation more reliable.
Pulse backfill system:
Three backfilling modes can be selected: slow, medium and fast, which avoids the problem of effective substances being blown away and lost due to the aeration of granular and flocculent materials after freeze-drying.
Manual automatic mode selection:
Manual mode is used to explore process parameters (strong human intervention), automatic mode is used in the mature stage of the process, one-click operation, simple and convenient
Data recording and analysis system:
Through the PC host computer, all operating parameters and background parameters of the equipment can be recorded in real time, and all parameter action changes and action changes of operating components can be recorded.
Freeze-drying process recipe storage function:
The host can store 60 sets of fixed or user-defined freeze-drying process recipes, and the PC can store >10,000 programs (depending on the computer hard drive)
Eutectic point test function:
The eutectic point temperature of the product can be tested offline and online, and the spectrum can be analyzed manually or automatically (optional)
Remote control system:
The operating status of the device can be monitored remotely, and the device can be monitored in real time via WiFi or the cloud.
Mobile phone monitoring system:
You can monitor the operating status of the equipment with your mobile phone, which is simple and convenient (optional)
Cold trap defrost system:
The hot air defrosting method has high safety performance; the defrosting speed is fast, which solves the inconvenience caused by traditional immersion defrosting.
DBCO-NHCO-PEG4-NHS ester is a PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-NHCO-PEG4-NHS ester is a PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA(not include miRNA) from a single sample (cells, soft tissue, plant sample)
Applications
RT-PCR, cDNA synthesis, PCR andsecond-generation sequencing, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues
Sample amount
Soft Tissue: < 30mg, Cell: <1 x 107, Plant: <100mg
Yield
DNA: 1 – 20 μg, RNA: 3 – 100 μg
Principle
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R511102
R511103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer DW1
30 ml
150 ml
Buffer RW1
30 ml
150 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Buffer AE
10 ml
50 ml
Storage and Stability
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
