Brevetoxins (PbTx) are a class of cyclic polyether compounds produced by certain algae such as Karenia brevis. K. brevis can produce Brevetoxins in large quantities during an algae bloom which then pose a major health threat and are important to monitor and mitigate. Brevetoxins are the causative agent of Neurotoxic Shellfish Poisoning (NSP).
FDA and EPA safety levels in regulations and guidance – 0.8 mg/kg for clams, mussels, oysters, and whole and roe-on scallops, fresh, frozen, or canned. – National Shellfish Sanitation Program Guide for the Control of Molluscan Shellfish.
Other Products
D3129 HiPure Insect DNA Kit
Product Info
Document
Product Info
Introduction
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from <10 mg insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
Midi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
<10 mg
Elution volume
≥15μl
Time per run
≤30 minutes
Liquid carrying volume per column
800μl
Binding yield of column
100μg
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Fast – several samples can be extracted in less than 30 minutes
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D312902
D312903
Purification Times
50
250
HiPure DNA Mini Columns I
50
250
2ml Collection Tubes
50
250
Buffer ITL
30 ml
120 ml
Buffer IL*
30 ml
120 ml
Buffer GW1*
22 ml
110 ml
Buffer GW2*
20 ml
2 x 50 ml
Proteinase K
24 mg
120 mg
Protease Dissolve Buffer
1.8 ml
15 ml
Buffer AE
15 ml
60 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Document
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from bacteria culture
Applications
RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Bacteria culture
Sample amount
Bacteria: <10^9
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Fast – several samples can be extracted in 30 minutes
High purity – the purified RNA can be directly used in various downstream applications
High recovery – RNA can be recovered at the level of PG
Good repeatability – silica gel column purification technology can obtain ideal results every time
Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
Sufficient components – the kit contains carried lysozyme and glass beads
Kit Contents
Contents
R418101
R418102
R418103
Purification Times
10 Preps
50 Preps
250 Preps
gDNA Filter Mini Columns
10
50
250
HiPure RNA Mini Columns
10
50
250
2ml Collection Tubes
20
100
500
Glass Beads (0.1-0.6mm)
10 g
30 g
150 g
Plastic spoon
2
4
10
Lysozyme
20 mg
90 mg
400 mg
Protease Dissolve Buffer
1.8 ml
1.8 ml
10 ml
Buffer TE
1.8 ml
1.8 ml
5 ml
Buffer STL
5 ml
20 ml
90 ml
Buffer RLC
10 ml
30 ml
150 ml
Buffer RW1
10 ml
50 ml
250 ml
Buffer RW2*
5 ml
20 ml
2 x 50 ml
RNase Free Water
1.8 ml
10 ml
30 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.
Document
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
