The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
Detail
Introduction
The RNA Clean Beads use paramagnetic bead technology for high-throughput purification of RNA or cDNA from in vitro applications such as transcription, antisense RNA (aRNA) amplification and RNA and cDNA probe synthesis. The resulting purified product can be used in the following applications: PCR and RT-PCR, probes for microarray or macroarray, RNase protection assays, transfection for RNAi experiments and cDNA synthesis and labeling.
Details
Specifications
Features
Specifications
Main Functions
Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen RNAClean XP)
Applications
Advanced applications, such as sequencing, genechip, fluorescence quantification, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
RNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount
20-100µl
Recovery
90%
Elution volume
≥20μl
Operation time
≤50 minutes
Principle
RNA Clean utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads. Excess oligonucleotides, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
High recovery – recovery of RNA up to 90%
High throughput – using magnetic beads purification technology
Store at 4℃ upon arrival, for up to 12 months. For best results shake the reagent well until all of the beads are completely in suspension and aliquot RNAClean Beads into RNase free containers. Do not pour remaining reagent back into the storage container. Mix RNAClean Beads well before use. The reagent should appear homogenous and consistent in color.
DO NOT FREEZE.
Other Products
Malaria TaqMan PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for Malaria
Available in TaqMan format for analysis
Malaria is a mosquito-borne infectious disease and considered one of the leading causes of death in the world. Malaria is a fatal tropical disease that is caused by a parasite knows as Plasmodium. It is usually spread through the bite of an infected female mosquito. 300-500 million new infected cases are estimated every year with 1.5-2.7 million deaths worldwide. Most of the malaria-related deaths occur in sub-Saharan Africa. . Although blood is the sample most frequently used to make a diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens
Malaria TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Malaria TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
1. User-Friendly Design: The chamber lid and tabletop are covered with heat-insulating resin materials, providing both protection against burns and an aesthetically pleasing appearance. The upward-opening flip-top door saves space, while the rounded corners prevent injury. The specially designed steam discharge and collection system minimizes steam burn risks and enhances operational convenience.
2. Interlock Mechanism and Safety Protection: The safety interlock system is designed to be more sensitive and secure. The chamber lid cannot be opened if there is pressure or steam inside the chamber. The lid can only be opened when the pressure gauge reads “0” MPa. The sterilizer will not start heating if the chamber lid is not properly locked. These features enhance the overall safety of the machine.
3. Over-temperature and Heating Protection: The system continuously monitors the temperature inside the sterilization chamber. If the temperature becomes too high or heating is abnormal, power will be automatically cut off to prevent overheating.
4. Automatic Fault Detection System: The machine performs an automatic self-check when powered on. If a fault occurs during operation, an audible alarm will sound, and a fault code will be displayed.
5. Lid Closure Check System: The system automatically detects whether the chamber lid is securely locked. If the lid is not properly locked, the sterilizer will not start.
6. Leakage, Over-current, and Short-Circuit Protection: If there is leakage in the heating elements or other components, or if over-current or short-circuit conditions occur, the system will automatically cut off power to prevent user injury.
7. Water Shortage and Independent Dry-Burning Protection: Equipped with a low water level sensor and independent dry-burning protection device, the system can monitor the water level in the chamber in real-time to prevent dry burning and avoid false readings. If the low water level sensor fails, the overheat and dry-burning protection system will automatically activate, cut off power to the heating ring, and sound an alarm to ensure safety.
8. Dual Pressure Protection: The system includes both a mechanical safety valve and an electronic over-pressure protection system. If pressure becomes abnormal, the system will relieve pressure and cut off power while sounding an alarm.
9. Back end Safety Testing: The system can be periodically tested for safety and protective measures.
10. Over-pressure Testing: Regular testing ensures that the instrument’s over-pressure protection system can automatically cut off power or that the safety valve can open to relieve pressure.
11. Performance Testing: The instrument’s safety performance has been tested by national authorized inspection agencies, and a test report is available.
Technical Parameters
Model
HB65A Standard TypeHB65B Auto Water Replenishment TypeHB65C Auto Drying Type
HB90AStandard TypeHB90BAuto Water Replenishment TypeHB90CAuto Drying Type
HB110AStandard TypeHB110BAuto Water Replenishment TypeHB110CAuto Drying Type
Capacity
65L
90L
110L
Dimensions (L × W × H) mm
664×660×994.5
664×660×994.5
664×660×1200
Sterilization Chamber Dimensions (Diameter × Height) mm
Timer Pre-set Range: Set operating time (year, month, day, hour, minute), check current time (year, month, day, hour, minute), total sterilization cycles, fault occurrence time, and code.
Pressure Gauge Display Range
0～0.6Mpa
Pressure
Chamber Design Pressure: 0.35 MPa; Each product comes with a quality certificate and a certificate issued by the special equipment manufacturing supervision and inspection department.
Pressure Safety Protection
Dual independent pipeline measurement and control, mechanical and electronic dual pressure protection
Fully automatic internal exhaust (see instructions for exhaust temperature settings), built-in large-capacity steam condensate collection bottle and water tank
Door lock interlocking device; Cover closing check; Dry burn protection; Water level sensor monitoring; Electronic over pressure protection; Mechanical safety valve; Over temperature and heating protection; Over current and short circuit protection; Leakage protection; Cooling lock; Burn injury safety protection; Automatic fault detection system
Printer, cooling fan, item temperature sensor, USB output
Stainless Steel Baskets
(φ360×230)1pcs(φ360×200)1pcs
(φ360×200)1pcs(φ360×400)1pcs
(φ360×200)2pcs(φ360×400)1pcs
Note: The upper limits for cooling lock door temperature and exhaust temperature are related to the boiling point.
Other Optional Accessories
1. Dot Matrix Printer: Capable of printing temperature, time, F0 value, and pressure data. USB data export and curve plotting.
2. Stainless Steel Basket with Base.
3. Pressure Sensor
4. Stainless Steel Bucket
5. Item Thermometer
6. 3Q Validation Adapter
Document
Features and functions:
1. Large-diameter sterilization chamber：The 40 cm large-diameter sterilization chamber provides an exceptionally spacious environment to meet a wide range of sterilization needs.
2. Sterilization temperature：105-138℃.
3. Exhaust control ：After sterilization is complete, the exhaust valve can automatically open to release the exhaust at a pre-set temperature.
4. Verification interface: Provides temperature and pressure inspection ports for easy calibration.
5. Saturated steam monitoring：The system automatically monitors the expulsion of cold air to ensure a pure steam sterilization environment, guaranteeing optimal sterilization effectiveness.
6. Pressure performance：The chamber is designed to withstand a pressure of up to 0.35 MPa, and each unit is tested with a water pressure of up to 0.44 MPa. Advanced pressure protection ensures the safety load of the equipment.
7. Intelligent control system:The microcomputer chip-based intelligent control system monitors the temperature and pressure inside the sterilization chamber in real-time. The chamber lid can only be opened under safe temperature and pressure conditions. The system also provides fully automatic control of the sterilization process and allows for convenient maintenance, inspection, calibration, recording, and future upgrades.
8. Six preset programs:Includes six fixed programs for liquid sterilization and holding, solid sterilization and drying, agar melting and holding, and waste sterilization.
9. Memory storage system:Users can set their own parameters, create multiple programs, and access them at any time. The system allows for operation permissions to be set at three levels—user, administrator, and engineer—to facilitate effective use and management of the sterilizer.
10. Door-opening temperature: Each program can be set with a specific cooling lock door-opening temperature based on the thermal inertia of the sterilized items. This prevents users from opening the door when the items (especially liquid sterilized items) are still in an unsafe condition, thus avoiding burns.
11. Drying function: Thorough drying that meets CE standards, with a cooling lock mechanism.
12. One-touch inquiry function: Allows checking of current time, total number of sterilization cycles, fault occurrence time, and codes.
13. Scheduled start function: Allows you to set a start time so that the sterilizer automatically starts at the scheduled time.
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Document
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
