Introduction
MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Details
Specifications
| Features | Specifications |
| Main Functions | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
| Applications | Sequencing, gene chip and qPCR, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
| Sample amount | Appropriate |
| Elution volume | ≥15μl |
| Operation time | ≤50 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excessprimes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
- Wide application – DNA products, enzyme digestion system, PCR reaction
- High recovery – suitable for 100bp-30kb fragments
- High purity – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
Kit Contents
| Contents | BXP-5 | BXP-50 | BXP-500 |
| MagPure A3 | 5 ml | 50 ml | 500 ml |
Storage and Stability
MagPure A3 should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.DO NOT FREEZE.
