Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Discover more assay kits for enzyme activity measurement.
Data calculators are located in the Documents tab.
Advantages
Very cost effective
All reagents stable for > 2 years
Specific for endo-1,3:1,4-β-glucanase/lichenase
Simple, convenient, rapid assay
Well suited to automation
Malt flour standard and lichenase standard included
Document
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
2-(Propargyl-PEG4-amido)-1,3bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions.
Document
2-(Propargyl-PEG4-amido)-1,3bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions.
Cell membrane preparations containing nicotinic AChRs that are ligand-gated ion channels that form pores in cells plasma membranes, mediating fast signal transmission at synapses. Nicotinic AChRs are involved in a wide range of physiological processes, and can be either neuronal or muscle-type. The membrane preparations we have developed are suitable for receptor binding assays in which muscle type nicotinic AChRs are needed. The membranes are tested in several functional binding assays and quality testing criteria to meet binding specifications.
