Attogene’s Carbon Dioxide Enzymatic Assay Kit is a simple, direct method for measuring Carbon Dioxide levels in the environment. The assay uses a coupled enzyme assay to detect CO2 (as HCO3-) as follows. In the first step, the bicarbonate condenses with phosphoenol pyruvate to form oxalate (and phosphoric acid); this reaction is catalyzed by the enzyme Phosphoenolpyruvate Decarboxylase, PEPC. The oxalate is then enzymatically reduced by the enzyme Malate Dehydrogenase (using an NADH cofactor) to form malate and NAD+.
Other Products
N-(Propargyl-PEG2)-PEG3-N-Boc
Product Info
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Product Info
N-(Propargyl-PEG2)-PEG3-N-Boc is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The Boc group can be deprotected under acidic conditions. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose.
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N-(Propargyl-PEG2)-PEG3-N-Boc is a branched PEG linker with an alkyne group and a t-butyl ester. The alkyne group reacts with azide-bearing compound in coppper catalyzed azide-alkyne Click Chemistry reaction. The Boc group can be deprotected under acidic conditions. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose.
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.
Details
Specifications
Features
Specifications
Main Functions
Recover 100-400μl DNA/ RNA from PCR products / enzymatic reaction solution / or crude DNA / RNA
Applications
Automatic sequencing, enzyme digestion, PCR and labeling
The MagPure method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger toparamagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
High recovery efficiency – up to 80% DNA recovery
Kit Contents
Contents
D500301
D500302
D500303
Purification Times
50 Preps
500 Preps
5000 Preps
Buffer AL
10 ml
60 ml
550 ml
Buffer BD*
5 ml
25 ml
2 x 100 ml
MagPure RNA Particles
1.2 ml
12 ml
120 ml
RNase Free Water
5 ml
30 ml
250 ml
Storage and Stability
MagPure RNA Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
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A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. This kit can be easily used in manual and automated 96 or 384-well formats.
