[CD1020] SMOChem™ Deoxynucleotide (dNTP) Mix, 25 mM each (100 mM total), 500 µl
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The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 25 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Detail
Description
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 25 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Features
Ideal for PCR amplification and cDNA synthesis
Premixed solution
Nuclease and ribonuclease free
Applications
PCR amplification of DNA fragments
DNA fill-in reaction
DNA sequencing
Reverse transcription
One-step RT-PCR
Storage
-20°C for 36 months
Other Products
IVD6672 MagPure Pathogen DNA/RNA Kit
Product Info
Document
Product Info
Introduction
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant.
Applications
RT-PCR，PCR
Products
Pathogen DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant
Sample amount
200-300μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Fast – several samples can be extracted in 40 minutes by column method
High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
Contents
IVD6672-50
IVD6672
Purification Times
50 Preps
200 Preps
Bead Tube C
50
4 x 50
MagPure Particle
1.6 ml
7.0 ml
Proteinase K
24 mg
100 mg
Protease Dissolve Buffer
1.8 ml
6 ml
Buffer MLBN
30 ml
120 ml
Buffer MW1*
22 ml
53 ml
Buffer MW2*
20 ml
50 ml
Buffer NFW
10 ml
30 ml
Storage and Stability
MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Document
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Short term stability: 2-8oC, Long term stability: See individual component labels
Analyte:
D-Fructose, D-Glucose
Assay Format:
Spectrophotometer, Auto-analyzer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
2.5 to 150 µg of D-glucose plus D-fructose per assay
Limit of Detection:
6 mg/L
Limit of Quantification:
16 mg/L
Reproducibility (%):
< 5%
Reaction Time (min):
~ 15 min for Total Sugars
The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
”Ready to use” liquid stable formulation – no reconstitution needed
PVP incorporated to prevent tannin inhibition
Standard included
Suitable for manual and auto-analyzer formats
Quick Reference Guide available
Mega-Calc™ software tool is available for hassle-free raw data processing
Document
The D-Fructose/D-Glucose (Liquid Ready™) assay kit is a method for the specific measurement and analysis of D-Fructose/D-Glucose in wine, beverages, foodstuffs and other materials. Measurements can be performed separately or combined. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Glucuronoyl Esterase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Reproducibility (%):
~ 3%
Total Assay Time:
10 min
Application examples:
Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition:
Novel method
The Glucuronoyl Esterase Assay Kit provides a simple robust method for the measurement of glucuronoyl esterase. The assay kit employs a novel colourimetric reagent, termed GEUX3 that is highly specific for glucuronoyl esterase.
The Glucuronoyl Esterase Assay Kit provides a simple robust method for the measurement of glucuronoyl esterase. The assay kit employs a novel colourimetric reagent, termed GEUX3 that is highly specific for glucuronoyl esterase.
