The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
Other Products
DBCO-PEG6-NHS ester
Product Info
Document
Product Info
DBCO-PEG6-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG6-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Isolate high quality DNA from a broad variety of phage strains
High yields of total DNA
Fast and easy processing using a rapid spin-column format
No phenol or chloroform extractions or cesium chloride banding required
High yields of DNA recovered3-15 µg DNA from 106-1010 pfu/ mL of enriched phages
This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
3-15 µg DNA from 106-1010 pfu/mL of enriched phages
Time to Complete 10 Purifications
45 minutes
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
Details
Specifications
Features
Specifications
Main Functions
Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate
Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Advantages
Fast – several samples can be extracted in 40 minutes (after digestion)
High purity – the purified DNA can be directly used in various downstream applications
Good repeatability – silica technology can obtain ideal results every time
High recovery – DNA can be recovered at the level of PG
Kit Contents
Contents
D314401
D314402
D314403
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
HiPure DNA Plate
1
4
20
96 Well Plate (2.2ml)
1
4
20
1.6ml Collection Plate
1
4
20
0.8ml Collection Plate
1
4
20
2ml Bead Tubes
100
400
2000
Buffer SOL
100 ml
360 ml
2 x 900 ml
Buffer SDS
10 ml
36 ml
180 ml
Reagent DX
1 ml
1.8 ml
9 ml
Buffer PS
20 ml
80 ml
400 ml
Absorber Solution
20 ml
80 ml
400 ml
Buffer GDP
150 ml
500 ml
3 x 900 ml
Buffer GW2*
50 ml
100 ml
4 x 200 ml
Buffer AE
30 ml
120 ml
500 ml
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.
