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Cell Culture Plate 6 Wells

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Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.

Specifications: 6wells

12wells, 24wells., 48wells, 96wells

Detail

Cell Culture Plate 6 Wells

Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.

Specifications: 6wells

12wells, 24wells., 48wells, 96wells

PRODUCT FEATURES

  • The product is made of medical grade USP CLASS VI polymer polystyrene
  • The product is made under a 100,00- class dust-free manufacturing site
  • Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

  • Gamma radiation sterilization
  • Non-Pyrogenic, DNase/Rnase free.

Other Products

8x10ml PRP Centrifuge Rotor

Plasma/Serum Exosome Purification and RNA Isolation Kits

Overview

  • Purification and enrichment of intact plasma/serum exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
  • Versatile plasma/serum input volume
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes required
  • No precipitation reagents, nor overnight incubation required
  • Compatible with plasma/serum from most species
  • Pure exosomes are purified and are free from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin
  • The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Details

Supporting Data

Figure 1 / 5

Previous

Figure 1. Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
Figure 1. Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
Figure 2. Plasma/Serum Exosome Purification and RNA Isolation Mini Kit
Figure 3. NanoSight data showing intact exosomes purified from 1 mL and 10 mL plasma
Figure 4. Plasma/Serum Exosome Purification and RNA Isolation Midi Kit
Figure 5. Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit

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Kit Specifications
Minimum Plasma/Serum Input50 μL
Maximum Plasma/Serum Input1 mL
Size of Exosomes Purified40 nm – 150 nm
Size of RNA PurifiedAll sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume50-100 μL
Time to Complete 10 Purifications35 – 40 minutes
Average Yields*Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

ComponentCat. 58300 (50 preps)Cat. 58500 (25 preps)Cat. 58600 (15 preps)
Slurry E12.5 mL12.5 mL12.5 mL
ExoC Buffer8 mL8 mL2 x 8 mL
ExoR Buffer12 mL12 mL12 mL
Lysis Buffer A20 mL20 mL20 mL
Lysis Additive B2 mL2 mL2 mL
Wash Solution A38 mL18 mL18 mL
Elution Solution A6 mL6 mL6 mL
Mini Filter Spin Columns502515
Mini Spin Columns502515
Collection Tubes502515
Elution Tubes (1.7 mL)1005030
Product Insert111

4x100ml Swing Buckets Rotor