Introduction

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.

Details

Specifications

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Storage and Stability

Carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.

K-ARGA

SKU: 700004264

115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser)

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See more of our monosaccharide assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Very rapid reaction due to inclusion of galactose mutarotase (patented technology) 
• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).

ChIP-Seq Library Prep Kit Workflow

ChIP-Seq is the combination of chromatin immunoprecipitation (ChIP) with next generation sequencing. It is a powerful tool for the analysis of global transcription factors and other proteins in diseases and biological pathways, and characterization of histone modifications in a genome-wide level at single-base resolution. ChIP-Seq delivers whole genome level of functional profiling of global transcription factors, and provides better understanding of epigenetic modifications.

Three index types are available for the ChIP-Seq Library Prep Kit of the illumina platform:

Non-index (Cat.# 30032): Libraries do not have index.

Index (Cat.# 30034): Each index primer contains a unique 6-base index sequence can be used for identification. 48 samples can be pooled together. Index information can be downloaded here.

Unique dual index (Cat.# 30036): The ChIP-Seq library multiplexing for 96 samples is possible. Our unique 4-Base Difference Index System have 8 bases index length and at least 4 bases are different from each other for better library identification. Our unique dual indexing primers remove sequencing errors such as index hopping, index contamination, mis-assignment, and other errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34034).

Kit advantages:

• Super fast protocol
  • Library prep can be done in 1.5 hours
  • The hands-on time is only around 10 minutes
• Easy procedure
  • Ready-to-use master mix simplified the procedure
  • Less reaction components make it easy to setup reactions
• Reduced more than half of the beads cost
• Input ChIP DNA: From 5 ng to 400 ng

Comparison of library conversion efficiency under the same condition. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Comparison of aligned reads, aligned rate and duplication rate. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.

Data comparison: Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used. Sequencing peak regions are shown.

The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).