Cerillo’s Alto Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This powerful optical system provides flexible calibration modes, accommodating a wide array of workflows including ELISA and microbial growth curves with diverse starting points. This plate reader is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 450nm or 600nm wavelengths.
Detail
Cerillo’s Alto Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This powerful optical system provides flexible calibration modes, accommodating a wide array of workflows including ELISA and microbial growth curves with diverse starting points. This plate reader is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 450nm or 600nm wavelengths.
Other Products
DBCO-Sulfo-NHS ester
Product Info
Document
Product Info
DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Clinical tissues, cells, lymphocytes
Sample amount
Tissue: <20 mgCells: <5 x 106
Yield
2-50μg
Elution volume
≥30μl
Time per run
≤25 minutes
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
Advantages
Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – the whole extraction only takes 15-25 minutes
Nontoxic – no toxic phenol chloroform extraction is required in the extraction
Kit Contents
Contents
IVD4121
Purification Times
50 Preps
HiPure DNA Mini Column Ⅱ
50
HiPure RNA Mini Columns
50
2ml Collection Tubes
150
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Buffer
6 ml
Buffer RTL
40 ml
RNA Digestion Buffer
15 ml
Buffer RWC*
20 ml
Buffer RW2*
20 ml
Nuclease Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
The Giardia intestinalis TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component
Cat. DxTM43800 (24 rxns)
MDx TaqMan 2X RT-PCR Master Mix Dx
550 µL
Giardia intestinalis Primer & Probe Mix Dx
2 x 70 µL
Giardia intestinalis Positive Control Dx – 200,000 copies/μL
