Cerillo’s Alto Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This powerful optical system provides flexible calibration modes, accommodating a wide array of workflows including ELISA and microbial growth curves with diverse starting points. This plate reader is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 450nm or 600nm wavelengths.
Detail
Cerillo’s Alto Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This powerful optical system provides flexible calibration modes, accommodating a wide array of workflows including ELISA and microbial growth curves with diverse starting points. This plate reader is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 450nm or 600nm wavelengths.
Other Products
IVD6672C MagPure Pathogen RNA Kit
Product Info
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Product Info
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) detection.
Details
Specifications
Features
Specifications
Main Function
Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection.
Applications
RT-PCR，PCR
Products
Pathogen RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, sputum
Sample amount
200-300μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Fast – several samples can be extracted in 40 minutes by column method
High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
Contents
IVD6672C
Purification Times
200 Preps
2ml Bead Tube
4 x 50
MagPure Particle
7.0 ml
Proteinase K
100 mg
Protease Dissolve Buffer
6 ml
DTT （Powder)
2g
Buffer SDS
15 ml
Buffer MLBN
220 ml
DNase I
4 x 0.6 ml
DNase Buffer
60 ml
Buffer MW1*
53 ml
Buffer MW2*
50 ml
Buffer NFW
30 ml
Storage and Stability
MagPure Particles and Proteinase K, DNase I should be stored at 2–8°C upon arrival. However, short-term storage (up to 2 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
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This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin sample extract and calibrators are pipetted into the test wells followed by the Cylindrospermopsin antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Cylin-drospermopsin from the sample and Cylindrospermopsin antigen compete for binding to the Cylindrosper-mopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Cylindrospermopsin and free Cylindrospermopsin antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived.
Format:
• 96-well microtiter plate (12 test strips of 8 wells)
