Cerillo’s Co-culture Discovery Bundle is a ready-to-use Co-Culture System designed for real-time phenotypic measurements. You can collect data from individual microbial populations without the need to custom build a solution. Shipped with 6 plates that hold 18 duvets per plate and 216 duvets.
Cerillo’s Co-culture Discovery Bundle is a ready-to-use Co-Culture System designed for real-time phenotypic measurements. You can collect data from individual microbial populations without the need to custom build a solution. Shipped with 6 plates that hold 18 duvets per plate and 216 duvets.
This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using spin-column chromatography based on Norgen’s proprietary resin. The kit includes all the reagents needed to process sputum DNA. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.
A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae ;and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.
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| Kit Specifications | |
| Time to Complete DNA Isolation | < 1 hour |
| Average Yield of DNA from 1.0 mL* | 75 μg |
| Average Purity (OD260/280) | 1.8 – 2.1 |
* Average DNA yield will vary depending on the health status of the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Norgen’s Sputum DNA Isolation Kit contains ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
| Component | Cat. 46200 (25 preps) |
|---|---|
| Slurry D | 55 mL |
| Proteinase K in Storage Buffer | 0.6 mL |
| Solution WN | 4 mL |
| Wash Solution BE | 9 mL |
| Elution Buffer B | 8 mL |
| Mini Filter Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 150 mg plant and fungal tissue |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Fresh / frozen plant samples, dried plant / seed samples |
| Sample amount | Fresh / frozen plant samples: 50-150 mgDried plant / seed samples: 15-40 mg |
| Elution volume | ≥40μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Kit Contents
| Contents | D318702 | D318703 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer PAL | 60 ml | 200 ml |
| Buffer GWP | 60 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer GW2* | 20 ml | 50 ml |
| Buffer AE | 20 ml | 60 ml |
| HiPure DNA Mini Columns II | 50 | 250 |
| 2 ml Collection Tubes | 50 | 250 |
Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
| HiPure HP Plant DNA Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
| HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
