Cerillo Stratus Plate Reader Adapter

Product Description

Product Detail

Kit Storage and Term of Validity

 Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

 Term of Validity: 14 months

Isothermal Nucleic Acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room   temperature and constant temperature (generally 39℃~42℃), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex;perform a homology search and bind the target homology domain,at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation,the polymerase also binds to the 3′ end of the primer, initiating chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit EXO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for RNA isothermal rapid amplification kit(Fluorescent type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.05 | 0.1 | 0.2 | 0.4 | 2 ppb
• Incubation Time: 75 Minutes

Description

Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa.  Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.

This kit can be used for quantitative test of Microcystin in liquid samples such as water, wastewater, algal cultures, and solid samples such as fish.

Microcystin-LR

• Informational sign postings about HABs at recreational waters: < 6 μg/L
• Recreational public health advisory: 6 μg/L
• Elevated recreational public health advisory (e.g. no contact): 20 μg/L

EPA 10-day drinking water health advisories:

• Do Not Drink – 0.3 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
• Do Not Drink – 1.6 μg/L for school-age children to adults.
• Do not Use – 20 μg/L

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.05 | 0.1 | 0.2 | 0.4 | 2 ppb
Incubation Time: 75 Minutes

Overview

• Isolation of exosomal RNA molecules from urine samples
• Rapid and convenient spin-column protocol
• Isolate inhibitor-free urinary microRNA for most downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Optional protocol for purification of exosomal proteins for western blot analysis

This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 50 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for using hazardous chemicals like phenol.

Background

Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. These vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which can inform about the cell type from which the exosomes are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNases they can be efficiently recovered from biological fluids, such as urine.

Details

Supporting Data

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Kit Specifications
Minimum Urine Input	1 mL
Maximum Urine Input	10 mL
Size of RNA Purified	Small exosomal RNA species
Time to Complete Purification	~ 50 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 47200 (50 preps)
Slurry B1	18 mL
Binding Buffer A	20 mL
Lysis Buffer A	2 x 20 mL
Wash Solution A	38 mL
Elution Solution A	6 mL
Mini Filter Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1