Overview
- CE-IVD marked in accordance with EU Directive 98/79/EC
- Ideal for use in in vitro diagnostic workflows
- Fixative-free preservative, no cross-linking of DNA
- Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C.
- Preserve cf-RNA for 30 days at ambient temperature
- Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
- No plasma volume loss after shipping/transportation
- Prevent hemolysis allowing better separation of plasma
- Prevent apoptosis of blood cells and fragmentation of genomic DNA
- Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
- Vacuumed to draw 8.4 mL of blood in 10 mL tubes
- The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume.
These tubes are ideal for collecting and preserving cf-DNA and cf-RNA samples for in vitro diagnostic use for medical purposes.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes Dx preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C.
Workflow
NOTE: This product is not available for sale in the United States. For the research use only version, see Cat. 63950
Details
Supporting Data
Figure 1 / 7
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| Kit Specifications | |
| Tube Size | 16 x 100 mm |
| Tube Type | PET Tube |
| Blood Draw Volume | 8.4 mL in 10 mL Tubes |
| Preservative Volume | 1.6 mL |
| Recovered Plasma Volume | Approximately 6-7 mL (not affected by shipping) |
| Anticoagulant | Proprietary |
| Preservative | Non formaldehyde – Proprietary preservation agent |
| Length of cf-DNA Preservation | Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C |
| Length of cf-RNA Preservation | Up to 30 days at room temperature (15-25°C) |
| Length of CTCs Preservation | Up to 14 days at room temperature (15-25°C) |
Storage Conditions and Product Stability
- When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx are stable through the expiration date. See tube label for expiry date.
- Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx.
- Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes Dx for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
- Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
- Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 8 days when stored at 37ºC.
- Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx is stable for up to 30 days when stored at room temperature (15-25°C)
- Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes Dx are stable for up to 14 days at room temperature (15-25ºC).
| Component | Cat. Dx63950 (50 tubes) |
|---|---|
| cf-DNA/cf-RNA Preservative Tubes Dx | 50 |
| Product Insert | 1 |
| Flowchart | 1 |
