[CK1000] Champion™ E. coli Transformation Kit, 200 Rxn

Designed for those labs wishing to separate magnetic beads into rings.

The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation

SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot 

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

RLE500

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated bracket to help keep microplate in place and flat
• Made in USA 
• For Research use only

Compatibility

• Full- skirt PCR plates
• 1/2- skirt PCR plates 
• Un-skirted PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. SPTLabtech Firefly®, Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 150 µL 

Minimum  – 5 µL 

Designed for those labs wishing to separate magnetic beads into rings.

The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation

• Screening of Microcystins in water samples at 6 ppb or lower
• Format: 10 tests (5 tests/5 control)
• Water Sample Bottles
• Negative Control
• 1mL Syringe
• Sample Dilution Buffer also sold separately
• Sample Filters

Description

Attogene’s Microcystin Test Kit (Rapid – Recreational Water) can be used to detect microcystins in liquid samples; highly sensitive, rapid, robust screening kit for algae toxins microcystins and nodularins.

The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.

A tiered notification system which takes different actions based on different numeric thresholds for microcytin-LR concentrations in recreational waters has been developed. This is guidance that allows states to take various actions—such as posting information about harmful algal blooms (HABs), issuing a recreational public health advisory, or temporarily closing recreational waters through a no contact advisory—depending on the severity of the bloom event.

Microcystin-LR

Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L

Harmful Algae Bloom – NOAA

Screening of Microcystins in water samples at 6 ppb or lower
Format: 10 tests (5 tests/5 control)
Water Sample Bottles
Negative Control
1mL Syringe
Sample Dilution Buffer also sold separately
Sample Filters

Description 

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)₂₀ and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 

Features

• Contains all components for reverse transcription 
• High yield 
• Thermostable, up to 50°C, during first strand synthesis 
• High processivity, generating cDNA up to 8 kb 
• Reduced RNase H ribonuclease activity 

Application

• Generation of first strand cDNA from total RNA or mRNA.
• Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.

Storage

-20°C for 24 months 

High yield

High sensitivity

Thermostable, up to 50°C, during first strand synthesis

 

Contents

ComponentVolume    ExcelRT™ Reverse Transcriptase (200 U/μl) 100 μl      RNase Inhibitor (20 U/μl)100 μl      5X RT Buffer (DTT)500 μl      dNTPs Mix (10 mM each)200 μl      Oligo (dT)20 (50 μM)100 μl      Random Hexamers (100 μM)100 μl      DEPC-Treated H2O  1 ml x 2

Storage Buffer

Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol

RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol

5X RT buffer (DTT)

250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl₂ and 50 mM DTT

Storage

-20°C for 24 months 

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.