Overview
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
- Higher intensity reference bands at 500 bp and 1000 bp
- 2686 bp (pUC19) reference band for easy clone identification
The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
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CloneSizer 100 bp DNA Ladder (Cat# 11600) – 100 loads
Ladder Properties:
- Fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments
- Higher intensity reference bands at 500 bp and 1000 bp
- 2686 bp (pUC19) reference band for easy clone identification
| Fragment | Size (bp) | Mass (ng) |
| 1 | 2686 | 72 |
| 2 | 2000 | 53 |
| 3 | 1500 | 41 |
| 4 | 1200 | 42 |
| 5 | 1000 | 56 |
| 6 | 900 | 30 |
| 7 | 800 | 29 |
| 8 | 700 | 25 |
| 9 | 600 | 25 |
| 10 | 500 | 52 |
| 11 | 400 | 19 |
| 12 | 300 | 20 |
| 13 | 200 | 19 |
| 14 | 100 | 17 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
