Description
- Appearance: White/blue
- • Length of strip: 85mm
- • Width of strip: 5mm
- • Length of device: 140mm
- • Width of device: 15mm
Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM/FITC-labelled primer during amplification. Test line: anti-biotin, Control Line: GAM
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC/FITC and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 GAM.
Inquire about custom configurations: [email protected]
Kit Components
Features & Benefits
Attogene Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a lateral flow dipstick assay for detection of DNA and RNA products.
Formats (strep gold conjugate pad):
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification. Test line: anti-biotin, Control Line: GAM
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FITC and Dig labelled primers during amplification.: Test Line #1: anti FITC/FAM, Line #2: anti-Dig, Line #3 Biotin.
Inquire about custom configurations: [email protected]
Kit Components
Features & Benefits
100 Lateral Flow Dipsticks (4.5mm)
20 mL Assay running buffer
100 wells with support plate
Controls
Usages:
For pre-enrichment of Salmonella spp.
Principle:
Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; potassium dihydrogen phosphate and disodium hydrogen phosphate as buffer.
Formulation(per liter):
Peptone: 10g
Sodium chloride:5g
Disodium hydrogen phosphate:3.5g
Potassium dihydrogen phosphate: 1.5g
Final pH7.2 ± 0.2
How to use:
1. Suspend 20g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 for 15min, set aside.
2.Diluted and treated samples.
Quality control:
Item The name and number of strain Growth Colony Color
1 Salmonella typhi CMCC (B) 50071 good Cloudy broth
2 Salmonella typhimurium CMCC (B) 50115 good Cloudy broth
3 Paratyphoid Salmonella CMCC (B) 50093 good Cloudy broth
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) detection.
Specifications
| Features | Specifications |
| Main Function | Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection. |
| Applications | RT-PCR，PCR |
| Products | Pathogen RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, sputum |
| Sample amount | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Kit Contents
| Contents | IVD6672C |
| Purification Times | 200 Preps |
| 2ml Bead Tube | 4 x 50 |
| MagPure Particle | 7.0 ml |
| Proteinase K | 100 mg |
| Protease Dissolve Buffer | 6 ml |
| DTT （Powder) | 2g |
| Buffer SDS | 15 ml |
| Buffer MLBN | 220 ml |
| DNase I | 4 x 0.6 ml |
| DNase Buffer | 60 ml |
| Buffer MW1* | 53 ml |
| Buffer MW2* | 50 ml |
| Buffer NFW | 30 ml |
Storage and Stability
MagPure Particles and Proteinase K, DNase I should be stored at 2–8°C upon arrival. However, short-term storage (up to 2 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.