CO00015 Midstream Cassette 136x16mm

N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-acid) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.

N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-acid) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The terminal carboxylic acids can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.

• All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
  + Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback). 
  + Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
  + Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.   
  + Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried. Can be freeze-dried by us. Learn MoreFor research use and further manufacturing. Designed and manufactured under ISO13485.

---

Exemplary tetraplex PCR assay

Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).

5x Multiplex – robust PCR perforemance for a wide range of qPCR application

PlexTaq 5x qPCR Multiplex Master Mix contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents aptamer prevents false amplification and provides a fast start function. 

PlexTaq®´s formulation allows to use it also for direct PCRs from crude samples.

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.

Description

Attogene’s Lead Rapid Lab Lateral Flow Kit can be used for the screening of Lead in water and food samples at greater than or equal to 10 ppb in a laboratory setting.  Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.

Lead contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Lead ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Lead-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Lead Lateral Flow test gives results conforming of 10ppb or greater in 5-15 minutes.

This is a revolutionary product designed to make Lead testing in easy and affordable.  This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L).

Sample Collection:

1. Take a first-draw sample

Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.

2. Take a flush sample

If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.

3. Take sequential samples

If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.

---

Standards and Regulations and recommendations for Lead

• EPA: Drinking Water: 15ppb
• FDA: Juice: 50ppb
• EPA: Residential Soil: 400 ppm (play areas), 1200 ppm (non-play areas)
• CPSC: Paint: 90ppm
• FDA: Bottled drinking water: 5ppb

High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death.  Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.

Screening of Lead in water samples at 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes