Description
- Appearance: White/pink
- • Length of strip: 86mm
- • Width of strip: 4.5mm
- • Length of device: 136mm
- • Width of device: 16mm
500 Lateral Flow Cassettes (top and bottom)
Cassette Lettering: CT
Fits strips that are 86 mm x 4.5 mm
Perfect for development and product launch
Fits into companion foil pouch bags
Large quantities available
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
The kit uses linear dsDNA libraries (MGI platform) as input and enriches the circularized single-stranded DNA libraries. The circularization kit has a higher library circularization efficiency (25%) as compared to other vendors (7-15%).
NGS Library Circularization Kit workflow
Comparison of Library Circularization Efficiency
Kit features
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue and cells and plant |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue |
| Sample amount | Cells: 1 x 107Animal tissue: ≤20mgPlant tissue: ≤100mgYeast cell: ≤5 x 106 |
| Yield | 5-100μg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Advantages
Kit Contents
| Contents | R662201 | R662202 | R662203 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| Proteinase K | 24 mg | 50 mg | 240 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| RTL Lysis Buffer | 40 ml | 80 ml | 400 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 x 220 ml |
| Buffer RW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I shouldbe stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles and Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.
This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.
Cell Culture Plate 96 Wells
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells,
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells,
12wells, 24wells., 48wells, 96wells