Description
- Appearance: White/pink
- • Length of strip: 86mm
- • Width of strip: 4.5mm
- • Length of device: 136mm
- • Width of device: 16mm
500 Lateral Flow Cassettes (top and bottom)
Cassette Lettering: CT
Fits strips that are 86 mm x 4.5 mm
Perfect for development and product launch
Fits into companion foil pouch bags
Large quantities available
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 500 mg soil sample |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Forest soil, grassland soil, mining area soil, sediment and other samples |
| Sample amount | 500 mg |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
| Contents | R418302 | R418303 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Micro Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 500 |
| gDNA Filter Column | 50 | 250 |
| 2ml Beads Tubes | 50 | 250 |
| Buffer SOL | 30 ml | 150 ml |
| Buffer SDS | 4 ml | 15 ml |
| Buffer PHC | 30 ml | 150 ml |
| Buffer GDP | 40 ml | 150 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2 * | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
Buffer PHC should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolvedbefore use. Make sure that all buffers are at room temperature when used.
Experiment Data
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications – Spin Column | |
| Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Maximum Amount of Starting Material:Plant TissuesFungi (wet weight) | 100 mg 100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) | 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont’d) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL | 2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL | 25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | – | – | – | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | – | – | – |
| Spin Columns | 50 | 250 | – | – | – |
| Collection Tubes | 100 | 500 | – | – | – |
| 96-Well Plate | – | – | 2 | – | 2 |
| 96-Well Collection Plate | – | – | 2 | – | – |
| Adhesive Tape | – | – | 4 | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – | 50 | – |
| 96-Well Elution Plate | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Propargyl-PEG18-alcohol is a crosslinker that can react with azide compounds under the catalyzation of copper to form stable triazole linkage. The hydrophilic PEG units increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG18-alcohol is a crosslinker that can react with azide compounds under the catalyzation of copper to form stable triazole linkage. The hydrophilic PEG units increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.