
Description
- Appearance: White/pink
- • Length of strip: 86mm
- • Width of strip: 4.5mm
- • Length of device: 136mm
- • Width of device: 16mm
500 Lateral Flow Cassettes (top and bottom)
Cassette Lettering: CT
Fits strips that are 86 mm x 4.5 mm
Perfect for development and product launch
Fits into companion foil pouch bags
Large quantities available
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters | Details |
---|---|
Product Name | RNA Isothermal Amplification Kit NFO |
Manufacturer | Amp-future |
Storage Temperature | -20°C |
Kit Components | Enzymes, Buffers ,Reagents |
Packaging | 48 Tests/box |
Detection Limit | 500-1000copies/µL |
Shipping | ICE |
Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Description
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and high signal intensity when compared with Brand A. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Features
Storage
Protected from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and high signal intensity when compared with Brand A. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for normalization of each qPCR assay. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.
Clone | IHC412 |
Source | Mouse Monoclonal |
Positive Control | Colon |
Dilution Range | 1:200 |
Postmeiotic Segregation Increased 2 (PMS2) is a DNA repair protein involved in mismatch repair. Mutations and deficiencies in the PMS2 gene have been linked to microsatellite instability, and malignancies such as hereditary nonpolyposis colorectal cancer and endometrial cancer. Expression levels of the PMS2 protein may be useful as a screening tool for Lynch syndrome after a colorectal cancer diagnosis. Anti-PMS2 is recommended to be used as part of a panel along with antibodies against MLH1, MSH2, and MSH6.
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