Description
- Appearance: White/Blue with lens
- • Length of strip: 86mm
- • Width of strip: 5mm
- • Length of device: 140mm
- • Width of device: 15mm
Blood Urea Nitrogen Enzymatic Kit is a microplate-based colorimetric
assay for the determination of urea in serum samples produced from blood. Blood urea nitrogen (BUN) is an important marker for normal kidney and liver function. Elevation of BUN levels is often an indication of intestinal and kidney obstruction and cardiac failure. Decreased BUN levels are often associated with kidney and liver damage. BUN is also a very useful tool
for preclinical investigation of experimental drug formulations and BUN levels are commonly used to monitor and attenuate the toxic effects of experimental drug formulations in rodents.
Blood Urea Nitrogen Enzymatic Kit uses an enzyme-based assay to determine urea in liquid samples such as serum. The test is based on a highly proven method for urea determination. The Blood Urea Nitrogen Enzymatic Kit contains sufficient materials to test 42 samples in duplicate. The assay utilizes urease, a metabolic enzyme, to specifically detect urea in serum. The Blood Urea Nitrogen Enzymatic Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 8 ppm urea for serum. The linear range of the assay is 8 – 200 ppm analyte.
96 determinations
Rapid and simple method
Minimal sample prep
Highly accurate and reproducible
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
NGS DNA Fragmentation & Library Prep Kit Workflow
The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.
Three index types are available for the kit of the illumina platform:
Non-index (Cat.# 30026): Libraries do not have index.
Index (Cat.# 30028): Each of the index primers contains a unique index sequence of 6 bases. Library multiplexing for 48 samples is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30030): Library multiplexing for 96 samples is possible. With the unique features of our 4-Base Difference Index System, the index sequence is 8-base long and each index has 4 bases different from others. Our unique dual index primers effectively identify sequencing errors such as index hopping, mis-assignment of reads, and de-multiplexing errors etc. The unique dual index primers set consists of 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34028).
Kit features:
Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.
The library size is inversely correlated with the incubation time of step 1 at 20°C.
NGS data comparison: enzymatic shearing versus mechanical shearing
Enzymatic shearing
• DNA shearing and library prep: BioDynami NGS DNA Fragmentation & Library Prep Kit
Mechanical shearing
• DNA shearing: Covaris sonication
• Library prep: BioDynami NGS DNA Library Prep Kit.
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
Usages:
For the differentiation of Gram-negative bacteria on the basis of citrate utilization.
Principle:
Magnesium ions in various metabolic cofactors; ammonium dihydrogen phosphate to provide nitrogen; dipotassium hydrogen phosphate is the buffer; sodium citrate as a carbon source; agar as medium coagulant.
Formulation(per liter):
Sodium chloride 5g
Magnesium sulfate 0.2g
Ammonium dihydrogen phosphate 0.2g
Sodium ammonium phosphate 0.8g
Sodium citrate 2g
Agar 15g
Bromothymol blue 0.08g
Final pH 7.0 ± 0.2
How to use:
1.Suspend 23.3g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
500g