CO00021 Midstream Cassette 126x15mm, pink

Overview

• Adenovirus Purification from any input – cell fraction or media fraction
• Rapid purification within 2 to 4.5 hours
• No specialized equipment needed (ultracentrifuge not required)
• Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep
• Purify adenovirus cell pellet from 1 mL of input per prep
• Up to 25X sample concentration
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger.  Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.

Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. 

Details

Supporting Data

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Kit Specifications
Resin Binding Capacity (total per kit)	At least 3×1011 adenovirus particles as determined by qPCR At least 3×108 transducing units as determined by transduction assay
Input type	Cells, media
Input volume (Supernatant)	1 – 33.5 mL SN per prep (500 mL SN in total)
Input Volume (Cell pellet)	1 mL cell pellet per prep (15 mL in total)
Minimum elution volume	1.3 mL per prep
Time to complete purification	2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction	Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 67600 (15 preps)
Lysis Buffer S	5.5 mL
HL-SAN Nuclease	102 µL
Binding Buffer A	20 mL
Purification Solution C	60 mL
Purification Solution D	130 mL
Wash Solution C	2 x 130 mL
Slurry E	12.5 mL
Elution Buffer P (store at 4°C)	66 mL
Protein Neutralizer	4 mL
Elution tubes (1.7 mL)	50
Product Insert	1

Character of Yingtai laboratory scale freeze dryer

Small size, and light weight

Compact construction of tabletop freeze dryer

Suitable for small samples drying

Advantage of Yingtai 3kgs smallest lab scale freeze dryer 

3kg water capture capacity, which can meet the needs of general laboratory freeze-drying capacity

-70°C ultra-low temperature cold trap, which can capture conventional organic solvents

Direct-capture coil cold trap with higher efficiency and better ice-catching effect

The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry organic solvents

Large flat flange type silicone seal, effectively guarantee the vacuum tightness, no need to apply any auxiliary sealing silicone grease forever

One-button operation, the host and the vacuum pump are linked, no manual operation required

The Pirani vacuum sensor with automatic temperature compensation can accurately detect the vacuum degree in the chamber at each stage

Removable sample shelf with special holes for centrifuge tubes, making it easier to place and adjust the height

Various types of chambers and T-frame configurations can fully meet the user’s sample placement

Vacuum pumps and sample placement chambers can be freely combined according to user needs, making the configuration more flexible and convenient

Techinical parameter

Item No	BD1-3	BD1-6	BD2-6
24/h water capture capacity	3kg	6kg	6kg
Cold trap temperature	-70℃	-70℃	-90℃
Cold trap volume	5L	10L	10L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	φ 200mm stainless steel with 3-5 layers available	●Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional
vacuum pump	Standard configuration
Oil mist filter	Extreme vacuum 4 * 10-4mbar,with a pumping capacity of 78L~200L/optional pump of various types
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
anti-corrosive treatment	Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	6/optional	1-48 pieces/optional
Eutectic point detection system	Support/Optional
Data output analysis	Support/Optional
Power	0.6kw	1.1kw	0.5kw

Overview

• ASTM Type I Water
• Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
• Nuclease-free, protease-free, endotoxin-free
• Suitable for molecular biology applications (qPCR, NGS etc.)
• Suitable for cell culture applications
• Produced in an ISO certified GMP laboratory
• Water system is routinely monitored for compliance with QC standards

The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.

Product Specifications
pH	n/a (highly pure water does not contain enough ions for an exact pH determination.)
Recommended Storage	Room Temperature
Grade	Ultrapure (ASTM Type I)
Biological Activity	DNase-, RNase-, Protease-, Endotoxin- Free
TOCs	< 50 ppb
Resistivity	> 18 MΩ-cm
Treatment	Not DEPC-Treated
Quantity	100 mL, 500 mL, 1000 mL
Purification Methods	Activated charcoal, polyphosphate, reverse osmosis, electro-deionization, ion exchange, UV, ultrafiltration
Shipping Conditions	Room Temperature

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.