Microcystin are a class of hepatotoxins produced by blue-green algae such as Microcystis aeruginosa. Microcystin-LR is the most common of the over 50 different congeners. Cyanobacteria can produce microcystin in large quantities during an algae bloom which then pose a major threat.
This kit can be used for Congener-Independent quantitative test of Microcystin in liquid samples such as drinking, ambient and waste water and algal cultures.
Additional Quality Control, Calibration Verification, and Spiking Solution materials can be obtained separately in optional Supplemental Pack for Method 546 (catalog # EL2024-Q).
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L
EPA 10-day drinking water health advisories:
Do Not Drink – 0.3 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.
Do Not Drink – 1.6 μg/L for school-age children to adults.
Do not Use – 20 μg/L
Other Products
Floor Type High Speed & Low Speed Universal Refrigerated Centrifuge
Product Info
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Product Info
GL20 Technical Parameter：
Max. Speed
21000rpm
Max. RCF
30910×g
Max. Capacity
4×800ml
Time Range
1~9h59min
RPM/RCF Convert
Yes
Noise (dB)
≤ 60
Temperature
-20℃~40℃
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
±1℃
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
680×540×830mm
Net Weight(Kg)
170KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for GL20
Order No
Rotor
Rotor Max Speed(r/min)
Volume(ml)
RCF(×g)
G20-1
Swing Rotor
4000
4×800ml
3450
G20-2
Microplate rotor
4000
4×4×96 well
2940
G20-3
Microplate rotor
4000
2×4×96 well
3210
G20-4
Microplate rotor
4000
2×3×48 well
2300
G20-5
Swing rotor
4000
4×40×7ml vacuum tube
3270
G20-6
4000
4×40×5ml vacuum tube
2890
G20-7
4000
4×28×10ml vacuum tube
3130
G20-8
Swing Rotor
4000
Fat bottle
3830
G20-9
Fixed Rotor
16000
4×8PCR
15760
G20-10
Fixed Rotor
15000
6×8PCR
21420
G20-11
Fixed Rotor
16000
8×8PCR
17480
G20-12
Fixed Rotor
15000
12×8PCR
22930
G20-13
Fixed Rotor
15000
40×0.5ml
22920
G20-14
Fixed Rotor
21000
12×1.5ml
30910
G20-15
Fixed Rotor
16000
24×1.5ml
23440
G20-16
Fixed Rotor
14000
30×1.5ml
20800
G20-17
Fixed Rotor
13000
48×1.5ml
17930
G20-18
Fixed Rotor
16000
16×5ml
22020
G20-19
Fixed Rotor
16000
6×10ml
21500
G20-20
Fixed Rotor
15000
12×10ml
22680
G20-21
Fixed Rotor
13000
16×10ml
19490
G20-22
Fixed Rotor
13000
8×15ml
17790
G20-23
Fixed Rotor
11000
12×15ml
14330
G20-24
Fixed Rotor
5000
24×15ml
3500
G20-25
Fixed Rotor
5000
30×15ml
3830
G20-26
Fixed Rotor
14000
6×30ml
19060
G20-27
Fixed Rotor
13000
6×50ml(conical)
18840
G20-28
Fixed Rotor
13000
6×50ml(round)
18730
G20-29
Fixed Rotor
5000
12×50ml
3860
G20-30
Fixed Rotor
4000
24×50ml
2970
G20-31
Fixed Rotor
13000
4×85ml
18940
G20-32
Fixed Rotor
12000
4×100ml
14850
G20-33
Fixed Rotor
10000
6×100ml
11380
G20-34
Fixed Rotor
4000
12×100ml
2970
G20-35
Fixed Rotor
12000
24 capillaries
15800
G20-36
Swing Rotor
15000
4×5ml
19920
G20-37
Vertical rotor
16000
16×5ml
16450
G20-38
Vertical rotor
14000
8×30ml
19750
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Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
Bioprocessing with Salt Active Nucleases – High Salt Conditions
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Product Info
Bioprocessing with Salt Active Nucleases – High Salt Conditions
OverView
For SAN HQ, SAN HQ ELISA Kit, and now SAN HQ GMP
SAN HQ GMP is biochemically identical to SAN HQ but produced under GMP conditions.
Applications
Purification of biologics from residual nucleic acids in biopharma manufacturing
Purification of recombinant proteins and enzymes for research and diagnostic use
Removal of unwanted nucleic acids contamination in molecular biology reagents in challenging conditions
Reduction of viscosity in biological samples during production and automation
Vaccine manufacturing and viral vector preparation
DNA removal in high-salt lysates
SAN HQ – Peak performance at high salt conditions
Salt Active Nuclease High Quality (SAN HQ) is a Bioprocessing Grade nuclease developed as the most efficient solution for removal of both single and double stranded DNA and RNA at high salt conditions.
This nonspecific endonuclease has peak activity at salt concentrations between 400 – 700 mM (Fig. 1)
Non-enveloped viruses like Adenoviruses and Adeno-Associated Viruses (AAV’s) are inherently more robust with two distinct advantages: 1) They exhibit higher tolerance to additives like salt and detergents and 2) their production often involves the lysis of host cells, allowing for harvesting non-secreted vectors.
For Adeno-Associated Viruses (AAVs), which are often harvested from crude cell lysate, the high salt tolerance of SAN HQ is particularly beneficial. Salt is typically added to such lysates to reduce viral aggregation, facilitating more effective nuclease action to digest residual DNA.
SAN HQ’s is engineered for optimum activity in these high salt environments ensuring that you achieve unparalleled DNA removal without compromising the integrity of these robust viral vectors.
Key Benefits
Optimized Residual DNA Removal: Ensures efficient degradation of residual DNA in high-salt conditions, meeting stringent quality requirements for biologics and vaccines.
Boosted AAV Vector Purification: Enhances the purification process for adeno-associated viral vectors in high-salt conditions, improving quality and yield.
Streamlined Workflow: Eliminates the need for desalting stages, simplifying the bioprocessing protocol and saving time and resources.
Enable High-Throughput Processes: Facilitates scale-up and automation by working effectively in high-salt environments, increasing operational throughput.
Potential Surge in Virus Yield: Operates under conditions that may boost the titer yield of AAV production, potentially enhancing overall viral yield.
Economized Enzyme Usage: Reduces the need for excess enzyme and additional process adjustments, resulting in significant cost savings.
Minimized Risk of Process Disruptions: Offers reliable performance in various high-salt bioprocessing conditions, reducing the likelihood of disruptions due to enzyme inhibition.
Reliability: Provides consistent enzyme activity in challenging high-salt conditions, adding a layer of predictability and dependability to your operations.
Broader Applicability: Versatile enough to be used in a wide range of viral vector systems, expanding your research and production capabilities.
Enhanced Viral Stability: High-salt levels stabilize viral vectors, and SAN HQ operates effectively in these conditions, maintaining high yield and quality.
Host Cell Lysis: Facilitates efficient lysis of host cells in high-salt conditions, optimizing the harvest of both secreted and non-secreted viral vectors.
Key Features
High purity (≥ 98%)
No protease detected
Supplied with extended product documentation
Compatible with SAN HQ ELISA
The Challenge in Removing Host Cell Chromatin Impurities
In bioprocessing, the primary role of a nuclease is to efficiently digest and fragment host-cell DNA into sufficiently small pieces, facilitating its removal during downstream processing. While most nucleases can effectively degrade naked DNA into tiny fragments under optimal conditions—as demonstrated by M-SAN HQ and SAN HQ, which can digest dsDNA into fragments smaller than 6 nt—the reality in bioprocessing is more complex. (See fig. 5)
The DNA targeted for removal often exists as chromatin, embedded in a complex matrix containing remnants of the lysed host cell as well as large amounts of the therapeutic product.The product may or may not have an affinity for the chromatin you aim to remove.
High salt is often applied to mitigate issues like aggregation. The real challenge lies in a nuclease’s ability to efficiently fragment chromatin under these more complicated, high-salt, conditions—not merely degrading naked DNA under ideal circumstances.
SAN HQ ELISA kit is developed for the detection and quantification of SAN HQ and SAN HQ GMP. The kit is designed as a classical sandwich ELISA, with two monoclonal antibodies specific towards SAN HQ nuclease (fig 6).
Features
Sensitive: 0.4 – 25.6 ng/ml
Precise: RSD ≤ 15%
Accurate: 100% ± 15%
Stability: 12 months when stored between +2°C to +8°C
Document
For SAN HQ, SAN HQ ELISA Kit, and now SAN HQ GMP
SAN HQ GMP is biochemically identical to SAN HQ but produced under GMP conditions.
Transcription Factor E3 (TFE3) is a transcription factor that binds to the MUE3-type E-box sequences involved in TGF-β signalling. Anti-TFE3 staining is the most sensitive and specific indicator of Xp11 translocation renal cell carcinomas. Since alveolar soft part sarcoma (ASPS) is characterized by a specific chromosomal rearrangement resulting in a chimeric transcription factor (ASPSCR1-TFE3), this TFE3 IVD antibody is also a useful diagnostic tool for recognizing ASPS.
