
Description
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Exceptional value for money
Rapid detection of 2019-nCoV
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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Kit Specifications | |
Column Binding Capacity | 25 µg |
Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) | 8 µg 10 µg |
Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension | 20 mg 3 x 106 cells 150 µL 150 µL |
Time to Complete 10 Purifications | 80 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 24700 (50 preps) | Cat. 24750 (100 preps) | Cat. 24770 (250 preps) |
---|---|---|---|
Digestion Buffer A | 25 mL | 2 x 25 mL | 5 x 25 mL |
Buffer SK | 30 mL | 2 x 30 mL | 5 x 30 mL |
Wash Solution A | 18 mL | 2 x 18 mL | 5 x 18 mL |
Elution Buffer B | 30 mL | 2 x 30 mL | 5 x 30 mL |
Proteinase K | 12 mg | 2 x 12 mg | 5 x 12 mg |
Spin Columns | 50 | 100 | 250 |
Collection Tubes | 50 | 100 | 250 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 |
Product Insert | 1 | 1 | 1 |
Norgen’s Exosomal RNA Isolation Kit (for the extraction of RNA from EV’s that have been isolated using IZON’s qEV columns) constitutes an all-in-one system for the isolation of exosomal RNA from exosomes previously isolated using IZON’s qEV columns or concentrated using IZON’s EV Concentration kit. This kit allows for the isolation of RNA from intact extracellular vesicles (EVs) where the purification is based on spin column chromatography that employs Norgen’s proprietary resin.
The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PR, reverse transcription PCR, Nothern blotting, RNase protection and primer extension, and expression array assays.
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15–25°C). This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Cat. 68910 (50 Preps) |
---|---|
Lysis Buffer A | 2 x 30 mL |
Lysis Additive B | 7 mL |
Wash Solution A | 38 mL |
Elution Solution A | 6 mL |
Mini Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
NH-bis(PEG2-propargyl) is a multi-branched linker with two terminal propargyl groups and an amino group. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
NH-bis(PEG2-propargyl) is a multi-branched linker with two terminal propargyl groups and an amino group. The propargyl groups reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. The amino group is reactive with carboxylic acids, activated NHS esters, and carbonyls. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Email : hej@a3p-scientific.com
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