
Description
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Exceptional value for money
Rapid detection of 2019-nCoV
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Specifications
Features | Specifications |
Concentration | 40 mg/ml |
Appearance | Suspension of dark brown particles |
Surface functional group | Si-OH, Silanol |
Dispersibility | Monodisperse,spherical |
Particle size | 1.0-1.5 μm |
Preservation conditions | Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
Magnetic response speed | ~30 seconds |
Settling velocity | >3 minutes |
High salt mediated binding | >2M guanidine isothiocyanate, DNA recovery up to 80% |
Alcohol mediated binding | 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
DNase/RNase | Not detected |
DNA residue | Not detected |
Recommended application | Genomic DNA extraction, RNA extraction, viral nucleic acid extraction, circulating DNA isolation |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
CAT.No. | Product Name | Package |
C14120 | MagPure Particles G | 100 ml |
C14121 | MagPure Particles G | 400 ml |
C14122 | MagPure Particles G | 3 x 400 ml |
C14123 | MagPure Particles G | 10 x 400 ml |
Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
DDL6 4x1000ml Swing rotor for 4x500ml Blood Bags Refrigerated Centrifuge
DDL6 Blood Bags Refrigerated Centrifuge Application:
DDL6 Refrigerated centrifuge widely used in the field of blood bank station, pharmaceutical factory and laboratory, can hold 500ml triple, 400ml triple and 250ml triple blood bags.
DDL6 Blood Bags Refrigerated Centrifuge Features:
1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.
2. Digital display which indicates the program, speed, time, RCF and temperature.
3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.
4. There are 6 kinds of rotors for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
DDL6 Blood Bags Refrigerated Centrifuge Technical Parameters:
Max Speed:6000r/min | Max RCF:6600g |
Max Volume;4x1000ml | Power supply:AC220V,50HZ,15A |
Timer:1~9h/59min | Noise:≤56dBA |
Dimension:800x675x830mm | Net Weight:260KG |
Speed Accuracy:±20r/min | Package (W*D*H):920x800x1320mm |
Temperature range: | -20℃ ~ 40℃ |
DDL6 Blood Bags Refrigerated Centrifuge Matched Rotors:
Rotor No. | Rotor type | Max.Speed (rpm) | Max.Volume (ml) | Max.RCF (xg) |
No33097 | Swing out rotor | 4,000 | 4x1000ml (round/square cup ) | 4,060 |
No30221 | Angle rotor | 6,000 | 4x300ml | 5,390 |
No30222 | Angle rotor | 6,000 | 6x300ml | 5,660 |
No30223 | Angle rotor | 6,000 | 6x500ml | 6,600 |
The Corners of our Company____________________________________
Metal housing with 3 layers protection. Quiet noise, Less vibration & Fast cooling guarantee a perfect separation.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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