CP0101 Triple Wrapped Irradiated Plate – Nutrient Agar Plate

Overview

• Detection kits for ZIKV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.

ZIKV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

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Storage Conditions

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM62250 (100 preps)	Cat. TM62210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
ZIKV Primer & Probe Mix	280 μL	280 μL
ZIKV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

K-CellG5-4V

SKU: 700004272

Content:	(K-CellG5-4V) 120 / 240 assays (manual) / 480 assays (auto-analyser) or  (K-CellG5-2V) 60 / 120 assays (manual) / 240 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	endo-Cellulase
Assay Format:	Spectrophotometer, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	1.2 x 10-3 U/mL
Reproducibility (%):	~ 3%
Total Assay Time:	10 min
Application examples:	Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition:	Novel method

The K-CellG5-2V pack size has been discontinued (read more).

Cellulase Activity Assay Kit.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

View Cellulase Activity Assay Protocol.

View our complete list of assay kits for enzyme activities.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).