CP0101J Triple Wrapped Irradiated Plate-Nutrient Agar
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55mm*10 plates / bag
Detail
Introduction
Usages: For monitoring and detection of surface of equipment and personnel hygiene.
Advantage: 1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life. 2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability. 3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation. 4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit.. 5.This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.
Specifications: 55mm*10 plates / bag
Other Products
IVD4144 HiPure FFPE RNA Kit
Product Info
Document
Product Info
Introduction
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology, DNase
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE slice, FFPE embedded tissue
Sample amount
Tissue: <6 mgFFPE: <6 slices
Yield
1-20μg
Elution volume
≥15μl
Time per run
≤30 minutes (after digestion)
Liquid carrying volume per column
800μl
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA wasfinally eluted with low-salt buffer.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 60 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
IVD4144
Purification Times
50 Preps
HiPure RNA Micro Columns
50
2ml Collection Tubes
50
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Booster Buffer
1.5 ml
Buffer DPS
60 ml
Buffer FRL
15 ml
Buffer RLC
15 ml
Buffer RWC*
10 ml
Buffer RW2*
20 ml
RNase Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG24-TFP ester is a heterobifunctional PEG linker with an azide-reactive DBCO and an amine-reactive TFP ester that is less susceptible to hydrolysis than an NHS ester. The PEG linker improves the water solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.