
Introduction
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM006
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Enterobacter Sakazakii Agar
1000mL
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM006
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Enterobacter Sakazakii Agar
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
029040 Methyl Red Reagent
Usage:For Methyl Red Test.
Specification:10ml
10ml
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from insect tissue |
Applications | PCR, southern bolt and virus detection, etc |
Purification method | 96 well DNA plate |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Insect tissue samples |
Sample amount | |
Elution volume | |
Time per run | |
Liquid carrying volume per column | |
Binding yield of column |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Contents | D313901 | D313902 |
Purification Times | 1 x 96 | 4 x 96 |
HiPure DNA Plate | 1 | 4 |
2.2 ml Collection Plate | 1 | 4 |
1.6 ml Collection Plate | 1 | 4 |
0.5ml Collection Plate | 1 | 4 |
Seal Film | 8 | 32 |
Buffer ITL | 30 ml | 120 ml |
Buffer IL | 30 ml | 125 ml |
Buffer GW1 | 44 ml | 2 x 110 ml |
Buffer GW2 | 50 ml | 3 x 50 ml |
Proteinase K | 50 mg | 200 mg |
Protease Dissolve Buffer | 6 ml | 15 ml |
Buffer AE | 20 ml | 60 ml |
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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