CRM006 Chromogenic Enterobacter Sakazakii Agar

Overview

• Detection kits for HPV (High and Low Risk)
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s HPV (High and Low Risk) TaqMan PCR Lyophilized Kit is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s HPV (High and Low Risk) TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Introduction

Usages:

For the rapid detection and of coliforms and E. coli.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate were mixed occurrence of coliforms and E. coli enzyme corresponding specific reactions, hydrolysis of the substrate, the release of the color groups, in a pale yellow plates coliforms appears orange-red colonies while E.coli appears blue-green colonies.

Formulation (per liter):

Peptone 15.0g

Yeast extract powder 3.0g

Sodium chloride: 5.0g

Sodium lauryl sulfate: 0.1g

Agar: 12.0g

Mixed chromogenic substrate: 6.77g

Final pH 7.0 ± 0.2

How to use:
1. Weigh 41.9g of the product, adding , 1.0 L of distilled or deionized water, heated to boiling stir until completely dissolved, dispensing into flask, 115 autoclaved 10minutes.

2, Take 25.0g or 25.0mL of sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) ,shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.

3, observe the results.

Quality control:

This product appears light yellow after  pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.

Bacteria name            bacteria NO.       growth situation         feature

Escherichia coli          ATCC25922            good         blue-green colonies

Citrobacter              ATCC8090             good         orange-red colonies

Salmonella typhimurium   CMCC50115          good           colorless colonies

Enterococcus faecalis     ATCC29212           suppressed            —–

Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.

1000mL

Permagen’s Dual Volume 0.2 mL PCR Strip separator uses the same magnet configuration as our MSR812 rack above, however, when using the opposite side, the magnets are positioned towards the bottom of the tubes allowing low elution’s to be achieved.  Angled tube holes allow clean area at the front of the well for clean, fast aspiration without the concern of disturbing bead pellets Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads

SKU #

MSRLV08

Features

• Two racks built into one
• 50 µL – 0.2 mL volumes on one side
• 5 µL – 50 µL volumes on opposite side
• Sides are conveniently marked with volume recommendations
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy tip tracking down front side
• Rubber feet to help prevent slippage on both sides
• Stable, solid aluminum design
• Angled tube holes which allow the tubes to come close to magnets

Compatibility

• Up to 16 Individual PCR tubes per side
• Up to two, 8-strip PCR tube strips per side
• Any Magnetic Beads

Volumes

Maximum  – .2 mL 

Minimum  – 5 µL 

Permagen’s Dual Volume 0.2 mL PCR Strip separator uses the same magnet configuration as our MSR812 rack above, however, when using the opposite side, the magnets are positioned towards the bottom of the tubes allowing low elution’s to be achieved. Angled tube holes allow clean area at the front of the well for clean, fast aspiration without the concern of disturbing bead pellets Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads