CRM007 Chromogenic E. coli O157

Product Description

Amp-Future NFO Isothermal Amplification Kit, -20℃ Storage, 48 Tests/Box, 500-1000copies/µL Detection Limit, 5-20mins Test

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Description

Specifications

Clone	IHC132
Source	Mouse Monoclonal
Positive Control	Astrocytoma
Dilution Range	1:200

Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumours, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma.

Features:

Large capacity water capture freeze dryer

Tabletop medium-sized freeze dryer

Suitable for drying medium-sized samples

Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.

product description:

9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity

-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents

Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes

Direct-capture large coil cold trap, higher efficiency, better ice capture effect

Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water

The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents

One-click hot gas defrost, with defrost compressor protection program.

Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease

High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate

The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar

Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier

Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement

Optional configuration of eutectic point detection system and data export system.

The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient

Techinical parameter

9-12L Floor tabletop and floor standing one for lab usage

Tabletop	BD1-9	BD2-9	BD1-12	BD2-12
Floor standing	BH1-9	BH2-9	BH1-12	BH2-12
Water Capture capacity	9L		12L
Cold trap temperature	-70 C	-90℃	-70C°	-90℃
Cold trap volume	15L		20L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional
Vacuum pump	Extreme vacuum 4 * 10-4mbar, pumping capacity ≥ 200L/can be equipped with various types of pumps
Oil mist filter	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16  ISO-KF L-1.5m
anti-corrosive treatment	Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	1-48 ports/optional
Eutectic point judgment	Support/Optional
Data audit trail	Support/Optional
Power	0.9kw	1.5kw	1kw	1.6KW
Size	390*468*448mm	480*498*487mm