
Introduction
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM007
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: E. coli O157 Agar
1000mL
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM007
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: E. coli O157 Agar
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from blood, buffy coat, tissue and other samples |
Applications | Second generation sequencing, PCR, real time PCR, etc. |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
Sample amount | Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg |
Yield | 0.1 – 50μg |
Elution volume | |
Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Contents | IVD3102 |
Purification Times | 200 |
MagPure Particles | 5 ml |
Proteinase K | 100 mg |
Protease Dissolve Buffer | 10 ml |
Rnase A | 40 mg |
Buffer ATL | 60 ml |
Buffer AL | 60 ml |
Buffer BD* | 20 ml |
Buffer BW1* | 110 ml |
Elution Buffer | 30 ml |
Cat.No | Reagent | IVD3102-F-96 |
Proteinase K | 50 mg | |
Protease Dissolve Buffer | 6 ml | |
RNase A | 20 mg | |
Buffer ATL | 30 ml | |
Buffer AL | 30 ml | |
96-Tip | 1 | |
Sample plate (DW Plate) | 450µl Buffer BD(Ethanol Added) | 1 |
Wash 1 Plate (DW Plate) | 600µl Buffer BW1(Ethanol Added) | 1 |
Wash 2 Plate (DW Plate) | 600µl Buffer BW1(Ethanol Added) | 1 |
Wash 3 Plate (DW Plate) | 750µl Buffer GW2, 20µl MagPure Particle | 1 |
Elution plate (DW Plate) | 80µl Elution Buffer | 1 |
Cat.No | Reagent | IVD3102-TL-06 |
Proteinase K | 50 mg | |
RNase A | 20 mg | |
Protease Dissolve Buffer | 6 ml | |
Buffer ATL | 40 ml | |
Buffer AL | 40 ml | |
AS-Tip | 12 | |
2.0ml V-bottom plate | Row 1/7:450µl Buffer BDRow 2/8:450µl Buffer BW1Row 3/9:450µl Buffer BW1Row 4/10:20μl Magpure Particle450μl Wash Buffer GW2 Row 5/11:450μl Wash Buffer GW2 Row 6/12:80µl Elution Buffer | 6 |
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
K-ACETGK
SKU: 700004257
110 mL of prepared reagent (e.g. 500 assays of 0.22 mL)
Content: | 110 mL of prepared reagent (e.g. 500 assays of 0.22 mL) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Acetic Acid |
Assay Format: | Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Increase |
Linear Range: | up to 1.8 g/L of acetic acid per assay |
Limit of Detection: | ~ 10 mg/L |
Reaction Time (min): | 8 min at 25oC or 5 min at 37oC |
Application examples: | Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Improved method |
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
As part of Megazyme’s overall commitment to providing the highest quality products, we have developed this acetic acid kit that provides a specific and rapid assay for use with auto-analysers. The kit assay is based on the conversion of NAD+ to NADH and therefore provides a positive reaction (increase in absorbance) which offers a more robust assay.
The reagents, as supplied, are stable for a minimum of 2 years and the prepared reagents are stable for a minimum of 1 week (on-board stability). In addition, the prepared reagents can be stored frozen for longer term stability (see K-ACETGK Booklet for more details).
View more of our acetic acid and organic acid assay kits.
Advantages
The Acetic Acid GK format test kit is for use with auto-analysers and is suitable for the specific measurement and analysis of acetic acid (acetate) especially in wines, fruit juices, beverages and food products.
Description
The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.
The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA. The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.
For Research Use Only
Features
Storage
Aliquot to avoid multiple freeze-thaw cycles
Protect fluorogenic probes from light
-20°C for 12 months
The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.
The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA. The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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