
Introduction
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM010
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Candida Agar
1000mL
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM010
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Candida Agar
This kit provides a rapid, single column method for the isolation and purification of total RNA (including miRNA) and proteins sequentially from a single sample of cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are both column purified in under 25 minutes using a single column.
Purified RNA is of a high quality and yield, and is suitable for NGS, RT-qPCR and microarrays.
This kit eliminates DNA efficiently using a gDNA removal column.
Proteins are eluted in buffer and are ready for downstream applications such as Western Blots, Mass Spec and ELISA. The proteins will not require precipitation, resuspending of pellets, or any further cleaning.
This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.
Protocol
Figure 1 / 2
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Kit Specifications | |
Maximum Column Binding Capacity | 50 μg for RNA |
Maximum Column Binding Capacity | 200 μg for Protein |
Maximum Column Loading Volume | 650 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Time to Complete 10 Purifications | 30 minutes |
Average Yields*: HEK 293 Cells (1 x 106 cells) HEK 293 Cells (1 x 106 cells) Liver (15 mg) Liver (15 mg) | 10-15 μg RNA 70-100 μg protein 30-35 μg RNA 100-150 μg protein |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 48200 (50 preps) |
---|---|
Buffer SKP | 40 mL |
Wash Solution A | 38 mL |
Elution Solution A | 6 mL |
Wash Solution C | 30 mL |
Binding Buffer A | 8 mL |
Elution Buffer C | 4 mL |
Protein Neutralizer | 4 mL |
Protein Loading Dye | 2 mL |
gDNA Removal Columns | 50 |
RNA/Protein Purification Columns | 50 |
Collection Tubes | 150 |
Elution Tubes (1.7 mL) | 100 |
Product Insert | 1 |
5 Layer Cell Factory Double Narrow Mouth
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.
PRODUCT FEATURES
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Huayi Cell Factory is a robust and easy-to-use cell culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.
DBCO-C6-Acid is an analog of DBCO-Acid with an extended 6-carbon atom spacer arm. The extended 6-carbon atom spacer arm improves its derivatization efficiency and the stability of the yielded conjugates. This spacer arm also improves solubility in organic solvents such as dichloromethane, chloroform, THF, and ethyl acetate. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-C6-Acid is an analog of DBCO-Acid with an extended 6-carbon atom spacer arm. The extended 6-carbon atom spacer arm improves its derivatization efficiency and the stability of the yielded conjugates. This spacer arm also improves solubility in organic solvents such as dichloromethane, chloroform, THF, and ethyl acetate. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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