CRM011 Chromogenic Schigella Agar

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ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.

Key Features:

• Novel substrate specificity
• RNA to DNA ligation
• High purity
• Detergent free
• Efficient ligation of DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the ligatable ends of DNA and RNA
• ATP dependent dsDNA ligase
• Utilizes Mg²⁺ or Mn²⁺

Suggested Applications:

• RNA capturing
• In vitro transcription  
• NGS library prep
• Transcriptome amplification
• miRNA detection/analysis
• RNA splint ligation

ArcticZymes RNA to DNA Ligase (ArcticZymes R2D Ligase™) is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.

Overview

• Detection kits for Epstein-Barr Virus (EBV)
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s EBV TaqMan PCR Lyophilized Kit is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s EBV TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM41050L (100 preps)	Cat. TM41010L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
EBV Primer & Probe Mix (Lyo)	280 μL	280 μL
EBV Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1

t-Boc-aminooxy-PEG4-propargyl is a click chemistry tool containing a propargyl group and t-Boc-aminooxy group. Propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. T-Boc-aminooxy can be deprotected under mild acidic conditions and then can react with an aldehyde or ketone group to form a linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

t-Boc-aminooxy-PEG4-propargyl is a click chemistry tool containing a propargyl group and t-Boc-aminooxy group. Propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to yield a stable triazole linkage. T-Boc-aminooxy can be deprotected under mild acidic conditions and then can react with an aldehyde or ketone group to form a linkage. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.