
Introduction
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM011
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Shigella Agar
1000mL
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM011
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Shigella Agar
The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.
Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:
– NEST 2 mL 96 Deep Well Plate, V bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 Well Plate Flat
– All Opentrons Flex tip racks
The Opentrons Flex Gripper GEN1 attaches to the gantry and is capable of moving labware across the deck and on or off modules such as the Magnetic Block or Thermocycler. It can also access the additional slots to the side of the main deck to replenish tips or labware. The gripper can be used with any pipette configuration.
Included with the gripper is a calibration pin that enables automatic calibration to ensure positional accuracy. Currently, the gripper is optimized for use with the following labware items:
– NEST 2 mL 96 Deep Well Plate, V bottom
– Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt
– NEST 96 Well Plate Flat
– All Opentrons Flex tip racks
These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS.
Cell-free circulating RNA, including exosomal RNA in plasma or serum, has the potential to provide biomarkers for certain cancers and disease states, and includes tumor-specific extracellular RNA in the blood. Exosomes are 40 – 100 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses, they can be efficiently recovered from biological fluids, such as plasma or serum.
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 μL to 200 μL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 μL to 25 μL.
This utilizes a two-column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 μL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
Figure 1 / 3
Click for expanded view
Kit Specifications | |
Sample Type | Plasma/Serum |
Anti-Coagulant (for Plasma)† | EDTA or Citrate |
Sample Volume Range | 50 to 200 μL |
Minimum Elution Volume | 10 μL |
Maximum Elution Volume | 25 μL |
Time to Complete 10 Purifications | 15 – 20 minutes |
Size of RNA Purified | All sizes, including miRNA and small RNA (<200 nt) |
Average Yields¥ | Variable depending on specimen |
† This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR
¥ Please check page 5 for Average Plasma/Serum Yields and Common RNA Quantification Methods
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Cat. 73700 (50 preps) | Cat. 73710 (20 preps) | Cat. 73720 (10 preps) |
---|---|---|---|
Lysis Buffer A | 2 x 20 mL | 100 mL | 1 x 130 mL 1 x 30 mL |
Wash Solution A | 18 mL* | 1 x 38 mL* 1 x 18 mL* | 38 mL* |
Elution Solution A | 6 mL | 6 mL | 6 mL |
Elution Buffer F | – | – | 15 mL |
EXTRACLean Micro Spin Columns | 50 | – | – |
EXTRACLean Mini Spin Columns | – | 20 | 10 |
EXTRACLean Midi Spin Columns | – | 20 | – |
EXTRACLean Maxi Spin Columns | – | – | 10 |
Collection Tubes | – | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
Product Insert | 1 | 1 | 1 |
Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.
Biotin-PEG4-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while terminal alkynes are used in copper (I) click chemistry with azides to form stable triazoles with a target molecule. The inclusion of a PEG linker in this molecule improves its aqueous solubility.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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