
Introduction
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM014
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Listeria Agar
1000mL
Place of Origin: Guangdong, China
Warranty: 2 years
Customized support: OEM
Brand Name: HKM
Model Number: CRM014
Reagent grade: Biochemical Reagent
Form: Powder
Color: Light yellow
Type: Listeria Agar
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from blood, buffy coat, tissue and other samples |
Applications | Second generation sequencing, PCR, real time PCR, etc. |
Purification method | Polydisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
Sample amount | Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg |
Yield | 0.1 – 50μg |
Elution volume | |
Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Contents | IVD3102 |
Purification Times | 200 |
MagPure Particles | 5 ml |
Proteinase K | 100 mg |
Protease Dissolve Buffer | 10 ml |
Rnase A | 40 mg |
Buffer ATL | 60 ml |
Buffer AL | 60 ml |
Buffer BD* | 20 ml |
Buffer BW1* | 110 ml |
Elution Buffer | 30 ml |
Cat.No | Reagent | IVD3102-F-96 |
Proteinase K | 50 mg | |
Protease Dissolve Buffer | 6 ml | |
RNase A | 20 mg | |
Buffer ATL | 30 ml | |
Buffer AL | 30 ml | |
96-Tip | 1 | |
Sample plate (DW Plate) | 450µl Buffer BD(Ethanol Added) | 1 |
Wash 1 Plate (DW Plate) | 600µl Buffer BW1(Ethanol Added) | 1 |
Wash 2 Plate (DW Plate) | 600µl Buffer BW1(Ethanol Added) | 1 |
Wash 3 Plate (DW Plate) | 750µl Buffer GW2, 20µl MagPure Particle | 1 |
Elution plate (DW Plate) | 80µl Elution Buffer | 1 |
Cat.No | Reagent | IVD3102-TL-06 |
Proteinase K | 50 mg | |
RNase A | 20 mg | |
Protease Dissolve Buffer | 6 ml | |
Buffer ATL | 40 ml | |
Buffer AL | 40 ml | |
AS-Tip | 12 | |
2.0ml V-bottom plate | Row 1/7:450µl Buffer BDRow 2/8:450µl Buffer BW1Row 3/9:450µl Buffer BW1Row 4/10:20μl Magpure Particle450μl Wash Buffer GW2 Row 5/11:450μl Wash Buffer GW2 Row 6/12:80µl Elution Buffer | 6 |
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Overview
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus uberis is a gram-positive bacterium that is known worldwide as an environmental pathogen responsible for a high proportion of cases of mastitis in lactating cows and is also the predominant organism isolated from mammary glands during the non-lactating period. Often it is resistant to treatment and causes persistent high somatic cell counts without clinical mastitis.
Streptococcus uberis TaqMan PCR Kit, 100 reactions
Streptococcus uberis TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component | Cat. TM30850 (100 preps) | Cat. TM30810 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
S. uberis Primer & Probe Mix | 280 μL | 280 μL |
S. uberis Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
Product Description
Fast RNA Extraction Reagent: High Efficiency, Reliable Results
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a RNA extraction kit that provides the highest quality nucleic acid extraction. The RNA extraction kit is an automated nucleic acid extractor which allows for quick and easy RNA isolation. The reagent is in liquid form and has a shelf life of 12 months, making it a reliable and durable product. With the Nucleic Acid Extraction Reagent, Amp-future Bio provides the accuracy and reliability of a professionally designed RNA extraction kit.
Features:
Technical Parameters:
Property | Value |
---|---|
Form | Liquid |
Product Name | RNA Nucleic Acid Extraction Reagent |
Application | RNA Extraction |
Brand | Amp-future Bio |
Shelf Life | 12 Months |
Composition | 5ml*4 tubes |
Applications:
Amp-future Bio’s Nucleic Acid Reagent is an advanced automated nucleic acid extraction solution designed to efficiently extract RNA from biological samples while maintaining quality and accuracy. It is made in China and has a 12-month shelf life. It comes in a liquid form and is suitable for use in automated nucleic acid extractors, allowing for automatic and highly efficient extraction of nucleic acid from various sample types. The reagent is also designed to be easy to use, making it an ideal solution for laboratories that require fast and reliable results. This reagent is a great choice for any research or clinical laboratory that needs efficient and accurate nucleic acid extraction.
Support and Services:
Technical Support and Services for Nucleic Acid Reagent
We provide technical support and services for Nucleic Acid Reagents. Our experts are available to help you identify the right reagents for your application, answer any questions, and provide assistance on the use and optimization of these reagents. Our experts are also available to provide advice on troubleshooting and protocol optimization. We offer a range of services to make ordering and stocking of reagents easy and convenient.
We provide:
Please contact us for more information about our technical support and services for nucleic acid reagents.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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