Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
OT-2 HEPA Filter H14 Replacement
Product Info
Document
Product Info
This H14 filter is a replacement for use with the OT-2 HEPA Module GEN1. The HEPA Module enables you to run sensitive contamination-prone applications on the OT-2.
Document
This H14 filter is a replacement for use with the OT-2 HEPA Module GEN1. The HEPA Module enables you to run sensitive contamination-prone applications on the OT-2.
Propargyl-PEG13-alcohol can reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form stable triazole linkage. The hydrophilic PEG units increase the water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG13-alcohol can reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form stable triazole linkage. The hydrophilic PEG units increase the water-solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
Time Savings
Cost Efficient
High Sensitivity and Yield
Robust Enzyme
Available in 12 or 50 reaction size
Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.
The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
Storage Conditions and Product Stability Norgen’s microScript microRNA cDNA Synthesis Kit components should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
