Bring your assay kits to the next level with freeze-dried PCR beads.
LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.
LyoBeads contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very simple. Only a biological sample has to be added.
Detail
Bring your assay kits to the next level with freeze-dried PCR beads.
LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.
LyoBeads contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which makes reaction setup very simple. Only a biological sample has to be added.
Storage: LyoBeads are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.
LyoBeads can be pre-dispensed in PCR-strips, PCR-plates, cartridges etc.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
D-Sorbitol, D-Xylitol
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
492
Signal Response:
Increase
Linear Range:
1.0 to 20 µg of D-sorbitol (or xylitol) per assay
Limit of Detection:
0.20 mg/L
Reaction Time (min):
~ 15 min
Application examples:
Diabetic foods (e.g. honey, jam and chocolate), dietetic foods, chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets, bakery products (e.g. desserts), marzipan, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by IFU and AIJN
The D-Sorbitol/Xylitol test kit for the determination Of D-Sorbitol And Xylitol In Foodstuffs, such as bakery goods, diabetic foods, chocolate, fruit, sweets and more.
Sorbitol is non-cariogenic and finds many dietetic applications, but can cause gastrointestinal problems in adults if large quantities are consumed (10-50 g per day). It has been safely used in processed foods for almost half a century and finds applications in the cosmetics and pharmaceuticals industries.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Each vial of sorbitol dehydrogenase is stable for > 2 months at 4oC after dissolution
No wasted diaphorase solution (stable suspension supplied)
Very competitive price (cost per test)
Reagents stable for > 2 years as supplied
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
Document
The D-Sorbitol/Xylitol test kit for the determination Of D-Sorbitol And Xylitol In Foodstuffs, such as bakery goods, diabetic foods, chocolate, fruit, sweets and more.
Isolation of exosomal RNA molecules from urine samples
Rapid and convenient spin-column protocol
Isolate inhibitor-free urinary microRNA for most downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Optional protocol for purification of exosomal proteins for western blot analysis
This kit provides a rapid spin column procedure for the isolation of exosomal RNA from urine samples. Users can simultaneously concentrate and isolate high quality exosomal RNA, including microRNA, for use in sensitive downstream assays such as RT-PCR, qRT-PCR, NGS, microarrays and more. The protocol can be completed in under 50 minutes. Urine volumes of 1 to 10 mL can be processed easily and rapidly. All sizes of RNA are recovered at an equal rate without the need for using hazardous chemicals like phenol.
Background
Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. These vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which can inform about the cell type from which the exosomes are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNases they can be efficiently recovered from biological fluids, such as urine.
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
