1.Widely used in the field of biochemistry、biological products、pharmaceutical factory and laboratory.
2. Brushless DC motor for model Cytoprep-1, free maintenance, no powder pollution, quick in speed up and down.
3. Smooth in operation, low noise and small vibration.
4. Frame is 3 tiers protection steel jacket, and with the stainless steel chamber, inside protective steel lagging which is simple-compact, light weight.
5. Microprocessor control, it can control rotate speed, and relative RCF, digital display which indicates the speed, time, RCF.
6. Automatically electric lid lock, super speed、over temperature protection and imbalance protection.
7. Power: AC 110V/220V, 50/60HZ, speed range:0-2800rpm, speed accuracy:± 20rpm
8. Emergency locks under the bottom of the centrifuge which help you open the lid when power off.
9. The centrifuge body is made of high-quality steel, safe and reliable.
Cytoprep-1 Cytology Technical Parameters:
Max speed：
4000rpm
Max RCF：
2170×g
Max volume
6×30ml
Noise：
≤55dBA
Timer
0～99min
Net weight：
28 KG
Dimension(HxDxW)：
280×528×370mm
Power supply
AC 220V 50HZ 2A
Speed accuracy：
±20rpm
Package
carton box or wooden box
Other Products
PACE® Genotyping Master Mix
Product Info
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Product Info
About
PACE Genotyping Master Mix uses a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals corresponding to genotypes. PACE compatible genotyping assays are comprised of two competitive allele specific forward primers (which differ in their terminal 3’ bases and unique 5’ tail sequences) and a common, reverse primer. PACE Genotyping Master Mix is supplied at 2x concentration and with ROX normalising dye at a range of levels to ensure compatibility with your qPCR instrument or reader.
Genotyping assay designs are available from 3CR Bioscience through our free PACE assay-design service; once designed, users can purchase assay primers independently or through 3CR Bioscience using our partial or full-assay validation service. PACE Genotyping Master Mix is also compatible with KASP™ and Amplifluor® marker assays.
REQUIRED COMPONENTS
qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays
For Research and Development purposes only. Not for diagnostic use.
Legal Information KASP™ is a trademark of LGC Biosearch Technologies Amplifluor® is a registered trademark of Merck KGaA
Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
Isolate full diversity of RNA from large RNA down to small and microRNAs
Eliminates PCR inhibitors including humic acids
High quality RNA for sensitive downstream application
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.
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Extracting Biological Insights from Stool
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Preserved, fresh, and frozen stool from humans and animals
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
600 μL
Time to Complete 10 Purifications
30 minutes
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Mycoplasma is the smallest and simplest prokaryotic organism. There is a risk of mycoplasma contamination during cell culture, and mycoplasma contamination of cells has become a common problem worldwide. Mycoplasma contamination may seriously affect the state of cells, change the gene expression and metabolic characteristics of cells, lead to slow cell growth, abnormal differentiation and death, and seriously affect cell function. Bacteria, yeast or mold contamination in cell culture can be seen under an optical microscope, but mycoplasma contamination is usually not visible under an optical microscope and must be detected by specific detection methods. Common methods for detecting mycoplasma contamination include mycoplasma isolation and culture, special biochemical tests such as ELISA and luminescence, and DNA fluorescent staining detection. Among the above detection methods, most of the operation steps are relatively cumbersome, the sensitivity is not high, the mycoplasma types cannot be distinguished, special instruments are required, or the time required is long. The qPCR method is relatively simple and convenient to operate, and the results can be obtained in 2 hours.
Features of MycoSEQ Mycoplasma Detection Kit
• Provide results in less than 2 hours. Good negative and positive controls. • No live mycoplasma required: The MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require the use of live mycoplasma for validation • Can be used with gDNA, inactivated mycoplasma, or live mycoplasma • Can be used as an alternative to the standard 28-day culture test • Can be used in conjunction with culture-based methods to provide preliminary results while awaiting the 28-day test results
The MycoSEQ Plus Mycoplasma Detection Kit is part of an integrated workflow for adventitious drug, impurity, and contaminant detection during biopharmaceutical manufacturing. The entire workflow includes the sample preparation kit, which provides a manual sample preparation method, which together with the qPCR analysis can provide results in 2 hours. (Please note that complex matrices may require additional upfront processing steps as described in the various available protocols.)
Contents and Storage
• Mycoplasma qPCR MIX, store at -15°C to -25°C, store at 2°C to 8°C after first use, protected from light. • Mycoplasma Positive Control, -15°C to -25°C. • Mycoplasma Negative Control, store at -15°C to -25°C, store at 2°C to 8°C after first use.
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Note: Price not include shipment & duty, contact us to get full quote. DuckyBio’s Mycoplasma PCR Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for detecting mycoplasma contamination in biological materials such as cultured cells. Designed and tested using criteria often applied toward rapid mycoplasma detection in biotherapeutic manufacturing cell culture lot release, the MycoSEQ Plus kit enables results that meet or exceed guidance on sensitivity and specificity expectations as described in European Pharmacopoeia (E.P. 2.6.7, 2007), US Pharmacopoeia (US63) and Japanese Pharmacopoeia. When used with a suitable sample preparation method, the MycoSEQ Plus kit can detect less than 5 CFU/mL.
