Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 10⁷) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.

Details

Specifications

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 40 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• High recovery – DNA can be recovered at the level of PG
• Sufficient components – lysozyme, protease K, RNase A and glass beads

Kit Contents

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.

Our 384-well PCR plates have a rigid fully-skirted frame which remains stable during thermal cycling.

About

Our 384-well plate has a rigid, fully-skirted frame which remains stable during thermal cycling.
The rigidity of the plate lends itself to automated and high-throughput PCR environments where robotic handling maybe in use.

The 2-component design reduces the risk of evaporation due to a poor sealing, commonly seen with 1-component PCR plates. 100% virgin medical grade polypropylene is used for the 384 wells, with a maximum capacity of 45 µL Certified free from DNase, RNase, nucleases and human gDNA.

Compatible with a wide range of PCR and qPCR applications, for a full list see ‘Plate Instrument Compatibility List’.

Available in clear or white well format.

About

PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.