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D-Sorbitol/Xylitol Assay Kit

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The D-Sorbitol/Xylitol test kit for the determination Of D-Sorbitol And Xylitol In Foodstuffs, such as bakery goods, diabetic foods, chocolate, fruit, sweets and more.

Detail

K-SORB

SKU: 700004339

58 assays (manual) / 580 assays (microplate) / 700 assays (auto-analyser) 

Content:58 assays (manual) / 580 assays (microplate) / 700 assays (auto-analyser) 
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:D-Sorbitol, D-Xylitol
Assay Format:Spectrophotometer, Microplate, Auto-analyser
Detection Method:Absorbance
Wavelength (nm):492
Signal Response:Increase
Linear Range:1.0 to 20 µg of D-sorbitol (or xylitol) per assay
Limit of Detection:0.20 mg/L
Reaction Time (min):~ 15 min
Application examples:Diabetic foods (e.g. honey, jam and chocolate), dietetic foods, chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets, bakery products (e.g. desserts), marzipan, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:Methods based on this principle have been accepted by IFU and AIJN

The D-Sorbitol/Xylitol test kit for the determination Of D-Sorbitol And Xylitol In Foodstuffs, such as bakery goods, diabetic foods, chocolate, fruit, sweets and more.

Sorbitol is non-cariogenic and finds many dietetic applications, but can cause gastrointestinal problems in adults if large quantities are consumed (10-50 g per day). It has been safely used in processed foods for almost half a century and finds applications in the cosmetics and pharmaceuticals industries.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

See our full range of alcohol test kits.

Scheme-K-SORB SORB Megazyme

Advantages

  • Each vial of sorbitol dehydrogenase is stable for > 2 months at 4oC after dissolution  
  • No wasted diaphorase solution (stable suspension supplied)  
  • Very competitive price (cost per test) 
  • Reagents stable for > 2 years as supplied 
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
  • Standard included 
  • Suitable for manual, microplate and auto-analyser formats

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IVD5435 MagPure Circulating DNA Maxi Kit

Introduction

This Kit is designed forpurification of high quality circulating DNA (cfDNA) from 1-6ml cell-free bodyfluids (such as plasma, serum). The purified DNA is suitable for direct use indownstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation circulating DNA from 1-6ml plasma, serum, body fluids
ApplicationsqPCR, NGS, etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeSerum, plasma
Sample amount1-6ml
Elution volume≥50μl
Time per run≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

  • Economy – less than 50% of the price of Qiagen and other imported products
  • Automatic – without labour
  • High purity – OD 260 / 280 : 1.7-1.9, OD 260 / 230 :1.5-2.0

Kit Contents

ContentsIVD5435
Purification Times50
MagPure Particles F14 ml
Carrier RNA310 μg
Proteinase K240 mg
Protease Dissolve Buffer15 ml
Buffer SDS15 ml
Buffer MLK500 ml
Buffer MAW1250 ml
Buffer MW2*50 ml
Elution Buffer60 ml

Storage and Stability

Proteinase K, Carrier RNA and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance.Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should beredissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

024048P1 Tryptic Soy Broth

Introduction

Usages:
For cultivating of bacteria.And sterility test of drugs and biological products.

Principle:
Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.

Formulation(per liter):
Pancreatic Digest of Casein            17g
Papaic Digest of Soybean              3g
Sodium chloride                     5g
Dipotassium hydrogen phosphate       2.5g
Glucose Monohydrate                2.5g
Final pH 7.3±0.2  

How to use:
1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

[CC0204] Champion™ 109 High, 24 tubes (Region Limited)

General information

Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.

Kit contents

  1. Champion™ Competent Cells
  2. pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
  3. Champion™ Transformation Protocol Card

Shipping condition

Throughout the shipping process, the temperature is maintained under -70°C. 

Storage and expiration 

Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.