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D2110 HiPure Gel DNA Micro Kit

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HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Detail

Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Details

Specifications

FeaturesSpecifications
Main FunctionsRecover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution
ApplicationsPCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification methodMicro spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeAgarose gel, PCR products, enzyme products
Sample amountAgarose gel: ≤200mgDNA products: ≤300µl (≤5µg)
Recovery100-5000bp:85%, 5-10kb:60-70%
Elution volume≥10μl
Time per run≤30 minutes(1-24 samples)
Liquid carrying volume per column800µl
Binding yield of column20µg


Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

  • High recovery efficiency – up to 80% DNA recovery
  • Low elution volume – elution volume can be as low as 10μl
  • General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
  • Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

ContentsD211002D211003
Purification Times100 Preps250 Preps
Buffer GDP60 ml125 ml
Buffer DW140 ml90 ml
Buffer DW220 ml50 ml
Elution Buffer20 ml30 ml
HiPure DNA Micro Columns100250
2 ml Collection Tubes100250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve

Other Products

D3311 SolPure Blood DNA Kit

Introduction

This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of blood samples. High-quality DNA can be purified from sample types including whole blood, buffy coat, bone marrow, body fluids in as little as 25 minutes. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from whole blood using economic salt out method
ApplicationsPCR, enzyme digestion, Southern hybridization,etc.
Purification technologySalting out method
Process methodManual (centrifugation or vacuum)
Sample typeAnimal tissue
Sample amountUnlimited
Elution volume≥100μl
Time per runVariation with sample amount

Principles

Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.

Advantages

  • Safe – without phenol chloroform extraction
  • Environment friendly – the reagents used are safe, non-toxic and without pollution
  • High molecular weight – the molecular weight of genomic DNA is about 50-150kb
  • High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
  • Unlimited sample size – solution type operation, sample volume can be adjusted at will
  • Cost performance – the most economical nucleic acid extraction technology

Kit Contents

ContentsD331101D331102D331103
Purification Volumes50 ml300 ml1000 ml
10 x Buffer RBC20 ml100 ml3 x 100 ml
Buffer WTL60 ml350 ml1000 ml
Buffer PPS20 ml120 ml350 ml
RNase Solution300 µl1.8 ml6 ml
Buffer TE10 ml30 ml120 ml

Storage and Stability

RNase Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

GeneAb™ Nerve Growth Factor Receptor (NGFR)

Description

Specifications

CloneIHC637
SourceMouse Monoclonal
Positive ControlBreast
Dilution Range1:200

PACE® ONESTEP RT-PCR MASTER MIX

ABOUT

PACE OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single reaction. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. PACE OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. PACE OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.