Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from <25 mg tissue, culture cells, FTA card
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissue or cultured cells
Sample amount	Animal tissue : <25mg, Cultured cells : <5 x 10⁶
Elution volume	≥30μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).

Advantages

Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
High purity – can be used for downstream applications such as multiplex and quantitative PCR
Fast – simplified process, extracting several samples in 20 minutes (after digestion)
Safety – no phenol orchloroform extraction, no alcohol precipitation

Kit Contents

Contents	D312102	D312103
Purification Times	50 Preps	250 Preps
Buffer ATL	15 ml	65 ml
Buffer DL	15 ml	65 ml
Buffer GW1	22 ml	110 ml
Buffer GW2	12 ml	50 ml
RNase A	10 mg	50 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml
HiPure gDNA Mini Columns	50	2 x 125
2 ml Collection Tubes	100	5 x 100

Storage and Stability

RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.