Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from <25 mg tissue, culture cells, FTA card |
| Applications | PCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissue or cultured cells |
| Sample amount | Animal tissue : <25mg, Cultured cells : <5 x 106 |
| Elution volume | ≥30μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins andother impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
- Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
- High purity – can be used for downstream applications such as multiplex and quantitative PCR
- Fast – simplified process, extracting several samples in 20 minutes (after digestion)
- Safety – no phenol orchloroform extraction, no alcohol precipitation
Kit Contents
| Contents | D312102 | D312103 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer ATL | 15 ml | 65 ml |
| Buffer DL | 15 ml | 65 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Buffer GW2 | 12 ml | 50 ml |
| RNase A | 10 mg | 50 mg |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
| HiPure gDNA Mini Columns | 50 | 2 x 125 |
| 2 ml Collection Tubes | 100 | 5 x 100 |
Storage and Stability
RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
