Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from small volume of blood, tissue and dry blood spots.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain |
| Applications | PCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissues, blood stain, urine, seminal stain and various forensic samples |
| Sample amount | Blood:1-100μl, Tissue:<10mg |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA).
Advantages
- Fast – several samples can be extracted in 20 minutes (after digestion)
- High purity – purified DNA can be directly used in various downstream applications
- High recovery – DNA can be recovered at the level of PG
- Good repeatability – silica technology can obtain ideal results every time
Kit Contents
| Contents | D312502 | D312503 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer ATL | 15 ml | 60 ml |
| Buffer AL | 15 ml | 60 ml |
| Buffer GW1* | 22 ml | 66 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| Carrier RNA | 310 μg | 2 x 310 µg |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 15 ml | 60 ml |
| HiPure DNA Mini Columns I | 50 | 2 x 125 |
| 2 ml Collection Tubes | 100 | 5 x 100 |
Storage and Stability
Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
Experiment Data
