Introduction
This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from yeast cultures |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Yeast culture |
| Sample amount | Bacterial culture: 1-1.5 ml |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).
Advantages
- Fast – several samples can be extracted in 40 minutes (after digestion)
- High purity – purified DNA can be directly used in various downstream applications
- Good repeatability – silica technology can obtain ideal results every time
- High recovery – DNA can be recovered at the level of PG
- Sufficient components – lysozyme, protease K, RNase A and glass beads
Kit Contents
| Contents | D314702 | D314703 |
| Purification Times | 50 Preps | 250 Preps |
| Hipure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Glass Beads (0.4~0.6mm) | 20 g | 90 g |
| Buffer SE | 30 ml | 150 ml |
| Lyticase | 1.8 ml | 5 x 1.8 ml |
| Buffer ATL | 30 ml | 150 ml |
| ReagentDX | 500 μl | 1500 μl |
| Buffer DL | 30 ml | 150 ml |
| Buffer GW1* | 13 ml | 66 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| Proteinase K | 12 mg | 60 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml |
| Buffer AE | 15 ml | 30 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.
