D6364 MagPure Stool DNA Kit

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MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Detail

Introduction

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 100-150mg stool samples
ApplicationsPCR, southern blot and enzyme digestion, etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeStool
Sample amount100-150 mg
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • Fast – several samples can be extracted in 60 minutes (after digestion)
  • High purity – unique adsorbent can completely remove inhibitory factors
  • High recovery – DNA can be recovered at the level of PG

Kit Contents

ContentsD636401D636402D636404
Purification Times48 Preps96 Preps400 Preps
MagPure Particles N1.7 ml3.4 ml14 ml
2ml Bead Tubes4896400
RNase A10 mg20 mg75 mg
Proteinase K24 mg48 mg180 mg
Protease Dissolve Buffer3 ml5 ml20 ml
Buffer ATL60 ml110 ml300 ml
PVP-101.2 g2.2 g6 g
Buffer PCI50 ml100 ml300 ml
Buffer MLE30 ml60 ml180 ml
Buffer GW1*22 ml44 ml132 ml
Elution Buffer20 ml20 ml60 ml

Storage and Stability

MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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