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D6364 MagPure Stool DNA Kit

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MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Detail

Introduction

MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 100-150mg stool samples
ApplicationsPCR, southern blot and enzyme digestion, etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeStool
Sample amount100-150 mg
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • Fast – several samples can be extracted in 60 minutes (after digestion)
  • High purity – unique adsorbent can completely remove inhibitory factors
  • High recovery – DNA can be recovered at the level of PG

Kit Contents

ContentsD6364
Purification Times400 Preps
MagPure Particles N14 ml
2ml Bead Tubes400
PVP-106 g
RNase A75 mg
Proteinase K180 mg
Protease Dissolve Buffer20 ml
Buffer ATL300 ml
Buffer PCI300 ml
Buffer MLE180 ml
Buffer GW1*132 ml
Elution Buffer60 ml

Storage and Stability

MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

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Place of Origin: Guangdong, China

Warranty: 2 years

Customized support: OEM

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Reagent grade: Biochemical Reagent

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Amyloglucosidase Assay Reagent

R-AMGR3

SKU: 700005031

200 assays per kit (4 vials)

Content:200 assays per kit (4 vials)
Shipping Temperature:Ambient
Storage Temperature:Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability:> 2 years under recommended storage conditions
Analyte:Amyloglucosidase
Assay Format:Spectrophotometer
Detection Method:Absorbance
Wavelength (nm):400
Limit of Detection:~ 2.5 U/mL
Reproducibility (%):~ 3%
Reaction Time (min):~ 15 min

High purity Amyloglucosidase Assay Reagent – 4 vials for the measurement of amyloglucosidase, for research, biochemical enzyme assays and in vitro diagnostic analysis. 

p-Nitrophenyl β-D-maltoside plus excess β-glucosidase.

Browse our complete list of assay kits and other reagent mixtures.