DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety and an azide reactive moiety. DADPS probe can be used in biomolecular labeling and proteomic studies. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DADPS (dialkoxydiphenylsilane) Biotin Alkyne probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety and an azide reactive moiety. DADPS probe can be used in biomolecular labeling and proteomic studies. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
D3187 HiPure HP Plant DNA Kit
Product Info
Document
Product Info
Introduction
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 150 mg plant and fungal tissue
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Broad spectrum – suitable for extracting DNA from various plant samples
Fast – several samples can be extracted in 30 minutes by silica technology
Good repeatability – silica gel column purification technology can obtain ideal results every time
Kit Contents
Contents
D318702
D318703
Purification Times
50 Preps
250 Preps
Buffer PAL
60 ml
200 ml
Buffer GWP
60 ml
200 ml
Buffer DW1
30 ml
150 ml
Buffer GW2*
20 ml
50 ml
Buffer AE
20 ml
60 ml
HiPure DNA Mini Columns II
50
250
2 ml Collection Tubes
50
250
Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation high pure total DNA from FFPE using high bind beads
Applications
PCR and viral DNA detection, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Paraffin embedded tissue samples
Sample amount
1-6 slices of 10-20μm
Elution volume
≥30μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High yield – most optimal process, recovery up to 90%
Economy – less than 50% of the price of Qiagen and other imported products
High purity – OD 260/280 : 1.7-1.9, OD 260/230 : 1.5-2.0
Kit Contents
Contents
D632301D
D632302D
Purification Times
48 Preps
96 Preps
MagPure Particles N
1.1 ml
2.5 ml
RNase A
10 mg
20 mg
Proteinase K
24 mg
48 mg
Protease Dissolve Buffer
3 ml
6 ml
Buffer DPS
60 ml
100 ml
Buffer ATL
15 ml
30 ml
Buffer BST1
30 ml
60 ml
Buffer BW1
13 ml
44 ml
Elution Buffer
15 ml
30 ml
Storage and Stability
Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.