DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
DBCO-amine TFA salt is a simple building block containing a DBCO moiety and an amine group. Amine is very reactive with NHS ester. DBCO is commonly used for copper-free Click Chemistry reactions.
Norgen’s cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen’s cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.
This product is for research use only and not for use in diagnostic procedures.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen’s cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.
Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.
Workflow
NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950
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| Kit Specifications | |
| Tube Size | 16 x 100 mm |
| Tube Type | PET Tube |
| Blood Draw Volume | 8.7 mL in 10 mL Tubes |
| Preservative Volume | 1.3 mL |
| Recovered Plasma Volume | Approximately 6-7 mL (not affected by shipping) |
| Anticoagulant | Proprietary |
| Preservative | Non formaldehyde – Proprietary preservation agent |
| Length of cf-DNA Preservation | Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C |
| Length of cf-RNA Preservation | Up to 30 days at room temperature (15-25°C) |
| Length of CTCs Preservation | Up to 14 days at room temperature (15-25°C) |
Storage Conditions and Product Stability
Biotin-PEG4-C1-alkyne is azide reactive biotinylation reagent, PEG4 arm increase aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Biotin-PEG4-C1-alkyne is azide reactive biotinylation reagent, PEG4 arm increase aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.