DBCO-C2-alcohol is a linker containing a DBCO moiety and a terminal primary hydroxyl group. DBCO group can react with azides in copper-free Click Chemistry reactions. The hydroxyl can react with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-C2-alcohol is a linker containing a DBCO moiety and a terminal primary hydroxyl group. DBCO group can react with azides in copper-free Click Chemistry reactions. The hydroxyl can react with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS.
Cell-free circulating RNA, including exosomal RNA in plasma or serum, has the potential to provide biomarkers for certain cancers and disease states, and includes tumor-specific extracellular RNA in the blood. Exosomes are 40 – 100 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes which depend upon the tumour cell type from which they are secreted. For this reason, exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses, they can be efficiently recovered from biological fluids, such as plasma or serum.
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 μL to 200 μL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 μL to 25 μL.
This utilizes a two-column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 μL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 μL to 100 μL.
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| Kit Specifications | |
| Sample Type | Plasma/Serum |
| Anti-Coagulant (for Plasma)† | EDTA or Citrate |
| Sample Volume Range | 50 to 200 μL |
| Minimum Elution Volume | 10 μL |
| Maximum Elution Volume | 25 μL |
| Time to Complete 10 Purifications | 15 – 20 minutes |
| Size of RNA Purified | All sizes, including miRNA and small RNA (<200 nt) |
| Average Yields¥ | Variable depending on specimen |
† This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR
¥ Please check page 5 for Average Plasma/Serum Yields and Common RNA Quantification Methods
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
| Component | Cat. 73700 (50 preps) | Cat. 73710 (20 preps) | Cat. 73720 (10 preps) |
|---|---|---|---|
| Lysis Buffer A | 2 x 20 mL | 100 mL | 1 x 130 mL 1 x 30 mL |
| Wash Solution A | 18 mL* | 1 x 38 mL* 1 x 18 mL* | 38 mL* |
| Elution Solution A | 6 mL | 6 mL | 6 mL |
| Elution Buffer F | – | – | 15 mL |
| EXTRACLean Micro Spin Columns | 50 | – | – |
| EXTRACLean Mini Spin Columns | – | 20 | 10 |
| EXTRACLean Midi Spin Columns | – | 20 | – |
| EXTRACLean Maxi Spin Columns | – | – | 10 |
| Collection Tubes | – | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 20 | 10 |
| Product Insert | 1 | 1 | 1 |
| Clone | IHC010 |
| Source | Mouse Monoclonal |
| Positive Control | Melanoma, Skin Melanocytes |
| Dilution Range | 1:200 |
SRY (Sex Determining Region Y)-Box 10 (SOX-10), also known as transcription factor SOX-10, is a nuclear transcription factor that acts in regulation of embryonic development and in the specification and differentiation of cells of melanocytic lineage. SOX-10 is diffusely expressed in neurofibromas and schwannomas, and mutations in the SOX-10 gene are linked to Waardenburg-Shah and Waardenburg-Hirschsprung disease. Anti-SOX-10 has been shown to be sensitive for conventional, spindled, and desmoplastic melanoma, and has been used to detect metastatic melanoma and nodal capsular nevus in sentinel lymph nodes.
Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva. Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications | |
| Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
| Average Yield from 0.25 mL of Saliva* | 2-5 μg |
| Average Purity (OD260/280) | 1.6 – 1.8 |
| Time to Complete 10 Purifications | 30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. RU55400 (50 preps) | Cat. RU62900 (192 preps) |
|---|---|---|
| Lysis Buffer F | 30 mL | 2 x 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | 2 x 1.1 mL | 8.5 mL |
| Elution Buffer B | 8 mL | 30 mL |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Adhesive Tape | – | 2 |
| Product Insert | 1 | 1 |
