Introduction

The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample)
Applications	SDS-PAGE electrophoresis and western blot, etc
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples
Sample amount	Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7

Principle

The Kit isolates total RNA from up to 10 ⁷ cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.

Advantages

• High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
• Fast – column method can complete the extraction of several samples in 30 minutes
• Safe – no phenol chloroform extraction required
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

Kit Contents

Contents	R521102	R521103
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Columns	50	250
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	100	2 x 250
Buffer RLC	50 ml	200 ml
Buffer GW1*	22 ml	66 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	50 ml	3 x 50  ml
RNase Free Water	10 ml	30 ml
Elution Buffer	10 ml	30 ml
Buffer ALO(5%SDS)	10 ml	30 ml

Storage and Stability

HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.

The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Overview

• Isolate all sizes of circulating DNA from plasma and serum samples
• Isolate Viral and Bacterial DNA
• Versatile plasma and serum input volumes
• Concentrate circulating DNA into small elution volumes
• Different elution strategies depending on the downstream application
• Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
• Compatible with Streck Cell-Free DNA BCT® Tubes
• Isolate DNA with high quality and quantity from milk
• Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.​​

Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.

Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.

Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)

Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.

Details

Supporting Data

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Kit Specifications
Minimum Plasma/Serum Input	50 μL
Maximum Plasma/Serum Input	400 μL
Minimum Elution Volume	100 μL
Time to Complete Purifications	< 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 50600 (50 preps)	Cat. 51200 (20 preps)	Cat. 51300 (10 preps)
Lysis Buffer D	65 mL	125 mL	3 x 125 mL
Slurry A1	–	6 mL	–
Slurry A2	12 mL	–	12 mL
Binding Buffer B	20 mL	6 mL	20 mL
Binding Buffer C	–	–	30 mL
Wash Solution A	2 x 38 mL	2 x 38 mL	3 x 38 mL
Elution Buffer B	8 mL	8 mL	15 mL
Mini Filter Spin Columns	50	20	–
Midi Filter Spin Columns	–	–	10
Midi Collection and Elution Tubes	–	–	20
Collection Tubes	50	40	10
Spin Columns	–	20	10
Elution Tubes (1.7 mL)	50	40	10
Product Insert	1	1	1

GL20 Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	4×800ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature	-20℃~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	680×540×830mm
Net Weight(Kg)	170KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL20

Order No	Rotor	Rotor Max Speed(r/min)	Volume(ml)	RCF(×g)
G20-1	Swing Rotor	4000	4×800ml	3450
G20-2	Microplate rotor	4000	4×4×96 well	2940
G20-3	Microplate rotor	4000	2×4×96 well	3210
G20-4	Microplate rotor	4000	2×3×48 well	2300
G20-5	Swing rotor	4000	4×40×7ml vacuum tube	3270
G20-6		4000	4×40×5ml vacuum tube	2890
G20-7		4000	4×28×10ml vacuum tube	3130
G20-8	Swing Rotor	4000	Fat bottle	3830
G20-9	Fixed Rotor	16000	4×8PCR	15760
G20-10	Fixed Rotor	15000	6×8PCR	21420
G20-11	Fixed Rotor	16000	8×8PCR	17480
G20-12	Fixed Rotor	15000	12×8PCR	22930
G20-13	Fixed Rotor	15000	40×0.5ml	22920
G20-14	Fixed Rotor	21000	12×1.5ml	30910
G20-15	Fixed Rotor	16000	24×1.5ml	23440
G20-16	Fixed Rotor	14000	30×1.5ml	20800
G20-17	Fixed Rotor	13000	48×1.5ml	17930
G20-18	Fixed Rotor	16000	16×5ml	22020
G20-19	Fixed Rotor	16000	6×10ml	21500
G20-20	Fixed Rotor	15000	12×10ml	22680
G20-21	Fixed Rotor	13000	16×10ml	19490
G20-22	Fixed Rotor	13000	8×15ml	17790
G20-23	Fixed Rotor	11000	12×15ml	14330
G20-24	Fixed Rotor	5000	24×15ml	3500
G20-25	Fixed Rotor	5000	30×15ml	3830
G20-26	Fixed Rotor	14000	6×30ml	19060
G20-27	Fixed Rotor	13000	6×50ml(conical)	18840
G20-28	Fixed Rotor	13000	6×50ml(round)	18730
G20-29	Fixed Rotor	5000	12×50ml	3860
G20-30	Fixed Rotor	4000	24×50ml	2970
G20-31	Fixed Rotor	13000	4×85ml	18940
G20-32	Fixed Rotor	12000	4×100ml	14850
G20-33	Fixed Rotor	10000	6×100ml	11380
G20-34	Fixed Rotor	4000	12×100ml	2970
G20-35	Fixed Rotor	12000	24 capillaries	15800
G20-36	Swing Rotor	15000	4×5ml	19920
G20-37	Vertical rotor	16000	16×5ml	16450
G20-38	Vertical rotor	14000	8×30ml	19750

Features

1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.