DBCO-C2-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-C2-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Cylindrospermopsin ELISA Kit
Product Info
Document
Product Info
Format: 96-well microtiter plate (12 test strips of 8 wells)
The Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin sample extract and calibrators are pipetted into the test wells followed by the Cylindrospermopsin antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Cylin-drospermopsin from the sample and Cylindrospermopsin antigen compete for binding to the Cylindrosper-mopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Cylindrospermopsin and free Cylindrospermopsin antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived.
Format:
• 96-well microtiter plate (12 test strips of 8 wells)
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year from the date of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution A should be stored at 4°C upon addition of RNase enzyme.
Component
Cat. 13300 (50 preps)
Cat. 46400 (250 preps)
Cat. 46500 (4 preps)
Cat. 46600 (20 preps)
Cat. 60300 (50 preps)
Cat. 63000 (192 preps)
Resuspension Solution AZ
12 mL
60 mL
20 mL
100 mL
12 mL
2 x 20 mL
Lysis Buffer N
40 mL
80 mL
40 mL
2 x 80 mL
40 mL
2 x 40 mL
Buffer TN
20 mL
130 mL
55 mL
2 x 130 mL
20 mL
1 x 55 mL 1 x 20 mL
Wash Solution E
12 mL
2 x 18 mL
–
–
–
–
Elution Buffer K
8 mL
30 mL
–
–
8 mL
2 x 8 mL
Wash Solution J
–
–
25 mL
3 x 25 mL
–
–
Elution Buffer J
–
–
24 mL
120 mL
–
–
RNase A
1 vial
1 vial
1 vial
1 vial
1 vial
1 vial
Magnetic Bead Suspension
–
–
–
–
1 x 1.1 mL
4 x 1.1 mL
Spin Columns
50
250
–
–
–
–
Collection Tubes
50
250
–
–
–
–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)
–
–
4
20
–
–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)
DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-Sulfo-NHS ester is a water-soluble sulfocated reagent containing DBCO moiety. It can be used for simple and efficient labeling of antibodies, proteins and any other primary amine-bearing macromolecules with DBCO moiety in 100% aqueous buffers. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.