NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

NH-bis(PEG4-Propargyl) is a bifunctional PEG compound containing two terminal alkynes that are joined together at a secondary amine. Terminal alkynes are reactive towards azides in copper (I) click chemistry to form stable triazoles with the target compound. The secondary amine joining the two arms can be used as a nucleophile such as in alkylation via reductive amination or in forming amides with carboxylic acids or activated NHS esters. The use of a central amine also allows for hydrogen bonding, further increasing this compound’s water solubility.

The TL 24 series of biological Microscope

TL 24 series biological microscope is equipped with 195 achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units

Details of TL24 series biological microscope

* Optical system: equipped with limited far flat field optical system, complete machine mildew prevention design and large field eyepiece, with beautiful appearance, clear imaging, broad vision, convenient operation and other characteristics, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units

* Observation head device: the cylinder can rotate 360 degrees, the displacement of the center of the mirror is 0.06mm; 30 tilting; the hinge double observation (the left and right systems are 2.0% different; the lens end position is 0.1mm at zero vision) and the third party connection port, pupil distance adjustment range of 55-75mm, fixed observation tube head is internal hexagon screw or select portable screw.

* Eyepiece configuration: random eyepiece WF10X / 18mm; the magnification accuracy of eyepiece shall not exceed ± 2%. There is a card slot on the eyepiece tube to lock and position the eyepiece to prevent falling. Binocular vision is adjustable, making binocular observation easier;

* Objective: Broadband coated ED-N195 pure copper achromatic objective; objective magnification accuracy not exceeding ± 5%; 4X, 10X, 40X (spring), 100X (spring, oil); objective error 10-40 Afocal 0.06mm; 40-100 Afocal 0.03mm

* Loading platform: triangular rail XY composite mechanical moving loading platform; 0.005 lateral to 5N horizontal force, corrosion resistance and wear resistance. Rectangular, size 110X120mm, stroke 7030mm, minimum read value 0.1 mm.

Specification

Specification configuration	TL24A	Binocular observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm
	TL24B	Three-eye observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm.
	TL24M	Three-eye viewing head, equipped with 5 million pixel imaging system CMOS camera
	TL24P	Three-eye viewing head, equipped with 10.1 inch integrated flat panel display
Eyepiece	Wide-angle eyepiece WF10X,field of view 18mm,optional WF16X,WF25X
Converter	Quadruple Revolving Converter
0bjective	195mm achromatic objective,4X,10X,40X,100X(oil)
Condenser	ABBE N.A.1.25Condenser with Iris Diaphragm &Filter
Stage	Double Layer Mechanical Stage,Size110mmx120mm,Moving Range :70mmx30mm
Focusing	Crude motion, micromotion and coaxial focal adjustment system with a minimum focal range of 0.02mm  Coarse and Fine Focusing
Light source	1W LED,with Rechargeable Battery,Type C USB cable
Attachments	Dust bag,Type C power cord

TL 24 series biological microscope is equipped with 195 achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units

Introduction

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 250-500mg soil sample
Applications	PCR, southern blot and enzyme digestion, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Soil
Sample amount	250-500mg
Elution volume	≥50μl
Time per run	≤70 minutes

Principle

This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• High concentration – maximum extraction of total DNA from soil samples
• High purity – purified DNA can be directly used in various downstream applications

Kit Contents

Contents	D635601	D635602	D635603
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure Particles	2.5 ml	5 ml	22 ml
2ml Bead Tubes	48	96	400
Buffer SOL	60 ml	100 ml	500 ml
Buffer SDS	6 ml	10 ml	50 ml
Reagent DX	1 ml	1 ml	5 ml
Buffer PS	10 ml	20 ml	90 ml
Absorber Solution	10 ml	20 ml	90 ml
Buffer GDP	70 ml	150 ml	2 x 350 ml
Buffer GW1*	22 ml	44 ml	220 ml
Elution Buffer	20 ml	20 ml	60 ml

Storage and Stability

MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.