DBCO-Maleimide is a sulfhydryl reactive reagent containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety into cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-Maleimide is a sulfhydryl reactive reagent containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety into cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
3,4-Dibromo-Mal-PEG4-amide-DBCO
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Product Info
3,4-Dibromo-Mal-PEG4-amide-DBCO is a PEG linker containing a dibromomaleimide group and a terminal DBCO group. The dibromomaleimide group allows for two points of attachement because both of the bromine atoms can be substituted. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. PEG linkers are hydrophilic moieties, therefore the attachment of a PEG linker to a compound increases it’s water solubility properties in aqueous media. Reagent grade, for research purpose.
Document
3,4-Dibromo-Mal-PEG4-amide-DBCO is a PEG linker containing a dibromomaleimide group and a terminal DBCO group. The dibromomaleimide group allows for two points of attachement because both of the bromine atoms can be substituted. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. PEG linkers are hydrophilic moieties, therefore the attachment of a PEG linker to a compound increases it’s water solubility properties in aqueous media. Reagent grade, for research purpose.
CleanAll DNA/RNA Clean-Up and Concentration Micro Kit
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Overview
Can be used for the clean up of both RNA and DNA from enzymatic reactions, labeling etc.
Purifies all sizes of RNA, from large mRNA down to microRNA (miRNA)
Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA
Removes endotoxins for transfection of injection ready RNA or DNA
Rapid and efficient spin-column format (20 minutes)
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions.
The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.
Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
Purify all sizes of DNA, from small PCR products, native or linearized plasmids, to genomic DNA. Using an alternative protocol, even oligonucleotides and smaller DNA fragments can be purified.
≥90% for RNA ≥90% for DNA 100 bp to 10 kbp ≥75% for DNA ≥ 10 kbp
* Applicable to smaller fragments or oligonucleotides with alternative protocol
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Lactose
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)
Limit of Detection:
1.62 mg/L
Reaction Time (min):
~ 10 min
Method recognition:
AOAC Method 2020.08
The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
