DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-NHCO-PEG13-NHS ester is a long chain PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines to form astable amide bond. The hydrophilic PEG13 arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis.
Features
5’→3’ DNA polymerase activity
No detectable 3’→5′ exonuclease (proofreading) activity
Generates PCR products with 3′-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
For direct loading into electrophoresis analysis
DNA bands can be visualized directly under UV or 470 nm blue light illumination
Applications
Routine PCR
Colony PCR
High throughput PCR
Amplification of DNA fragments up to 8 kb
Generation of PCR products for TA cloning
DNA labeling
Storage
Protected from light and avoid multiple freeze/thaw cycles 4°C for 6 months -20°C for 24 months
Document
The ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis.
Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-amine has a propargyl group and an amine group. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls, etc. The propargyl group can form triazole linkage with azide-bearing biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)
Content:
141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Acetic Acid
Assay Format:
Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
up to 30 μg/mL of acetic acid per assay
Limit of Detection:
10 mg/L (recommended assay format)
Reaction Time (min):
~ 15 min
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by EN, ISO, ICUMSA, IFU and MEBAK
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products.
See more of our acetic acid and organic acid test kits.
Advantages
No wasted ACS solution (stable suspension supplied)
PVP incorporated to prevent tannin inhibition
Very stable reagent when prepared for auto-analyser applications (> 3 days at 4oC)
Linear calibration up to 30 μg/mL of acetic acid in final reaction solution
Validated by the University of Wine, Suze la Rousse, France
Very competitive price (cost per mL of reagent)
All reagents stable for > 2 years after preparation
Document
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products.