DBCO-NHCO-PEG13-NHS ester

The Flex caddy allows the Thermocycler GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Thermocycler. Caddies and calibration adapters are specific to the type of module.

Note: This item does not include the Thermocycler Module. The module is available with or without the Flex Caddy and Calibration tool: Thermocycler Module GEN2

The Flex caddy allows the Thermocycler GEN2 to be installed on the Opentrons Flex deck and sit below the deck, enabling below-deck cable routing and labware placed on top of modules to remain closer to the deck surface. The calibration adapter is used to calibrate the location of the Thermocycler. Caddies and calibration adapters are specific to the type of module.

Note: This item does not include the Thermocycler Module. The module is available with or without the Flex Caddy and Calibration tool: Thermocycler Module GEN2

Overview

• Fast and easy recovery of DNA from agarose gel fragments
• High recovery of desired DNA
• Convenient spin column format
• DNA is ready for ligation, restriction digestion, sequencing and more

This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing.  The protocol can be completed in 20 minutes.  

Details

Supporting Data

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Kit Specifications
Column Binding Capacity	10 μg
Maximum Weight of Gel Slice	400 mg
Average Recovery	70-90%
Size of DNA Purified	100 – 15,000 bp
Minimum Elution Volume	30 μL
Time to Complete 10 Purifications	30 minutes

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 13100 (50 preps)
Binding Buffer G	80 mL
Wash Solution A	12 mL
Elution Buffer B	8 mL
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Propargyl-PEG13-t-butyl ester enables Click Chemistry reactions with azide-bearing compounds or biomolecules to form stable triazole linkages; copper is required as a catalyst. The t-butyl protection can be removed under acidic conditions. The PEG units improve the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG13-t-butyl ester enables Click Chemistry reactions with azide-bearing compounds or biomolecules to form stable triazole linkages; copper is required as a catalyst. The t-butyl protection can be removed under acidic conditions. The PEG units improve the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.