DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG4-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.
The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.
The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.
The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Do you require gDNA removal in applications other than RT-qPCR? Contact our support team for assistance in implementing dsDNase treatment in your workflow.
The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
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