DBCO-NHCO-PEG5-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG5-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media and can thus be used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Serum, plasma, acellular samples |
| Sample amount | 300μl |
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R662801 | R662802 | R662803 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles N | 1.7 ml | 3.5 ml | 17 ml |
| Buffer CFL | 6 ml | 12 ml | 60 ml |
| Buffer CPL | 1.8 ml | 3.5 ml | 20 ml |
| Buffer MGW1* | 30 ml | 60 ml | 250 ml |
| Buffer MW2* | 12 ml | 25 ml | 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Propargyl-PEG4-(CH2)3-CO2H is a linker containing a propargyl group at one end and a carboxylic acid at the other end. The carboxylic acid reacts with amine groups in the presence of activators (EDC or HATU). Under the catalyzation of copper, the propargyl group forms linkage with azide group of biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-(CH2)3-CO2H is a linker containing a propargyl group at one end and a carboxylic acid at the other end. The carboxylic acid reacts with amine groups in the presence of activators (EDC or HATU). Under the catalyzation of copper, the propargyl group forms linkage with azide group of biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
