DBCO-NHCO-PEG5-acid

K-MRGLUC

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Content:	1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Glucose
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance, Fluorescence
Wavelength (nm):	(Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570
Signal Response:	Increase
Linear Range:	(Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay) (Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay)
Limit of Detection:	(Fluorescence) 0.09 ug/mL (Absorbance) 0.25 ug/mL
Reproducibility (%):	(Fluorescence) ~ 6% (Absorbance) ~ 7%
Reaction Time (min):	(Fluorescence) 30 min (Absorbance) 30 min
Application examples:	Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.).

This product has been discontinued.

The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.

*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.

See more related mono/disaccharide assay kit products. 

Fluorometric/UV method for the determination of D-glucose content in a variety of samples.

Advantages

• Simple format
• Very competitive price (cost per test)
• Ability to run in fluorescence or absorbance mode
• User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Suitable for manual, microplate and auto-analyser formats
• All reagents stable for > 2 years

The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.

Character

High flatness sample shelf:

Silicone oil thermal conductive shelf, high-precision processing method of upper and lower clamping, shelf temperature uniformity can reach ±0.5°c

Integrated cavity:

Integrated chamber, the shelf and cold trap are located in the same chamber, the steam conduction is faster and the freeze-drying effect is better

Refrigerating and heating control system:

Using thermally conductive silicone oil with a high temperature range and precise PID control algorithm, the control accuracy can reach ±0.5°C. Sample shelf temperature range: -70°C~ +80°C

Freeze-drying endpoint testing system:

The pressure comparison method is used to determine the freeze-drying end point. The freeze-drying end point test can be automatically performed after the analytical drying stage to ensure that the moisture content of the material reaches the standard requirements.

Vacuum precise control and adjustment system:

The sublimation and analytical drying processes are controlled and adjusted with high precision to make the freeze-drying process warmer and the sample sublimation more reliable.

Pulse backfill system:

Three backfilling modes can be selected: slow, medium and fast, which avoids the problem of effective substances being blown away and lost due to the aeration of granular and flocculent materials after freeze-drying.

Manual automatic mode selection:

Manual mode is used to explore process parameters (strong human intervention), automatic mode is used in the mature stage of the process, one-click operation, simple and convenient

Data recording and analysis system:

Through the PC host computer, all operating parameters and background parameters of the equipment can be recorded in real time, and all parameter action changes and action changes of operating components can be recorded. 

Freeze-drying process recipe storage function:

The host can store 60 sets of fixed or user-defined freeze-drying process recipes, and the PC can store >10,000 programs (depending on the computer hard drive)

Eutectic point test function:

The eutectic point temperature of the product can be tested offline and online, and the spectrum can be analyzed manually or automatically (optional)

Remote control system:

The operating status of the device can be monitored remotely, and the device can be monitored in real time via WiFi or the cloud.

Mobile phone monitoring system:

You can monitor the operating status of the equipment with your mobile phone, which is simple and convenient (optional)

Cold trap defrost system:

The hot air defrosting method has high safety performance; the defrosting speed is fast, which solves the inconvenience caused by traditional immersion defrosting.

Parameter

Model	EX2	EX5	EX8	EX12
Freeze-area	0.24m²	0.38m²	0.96m²	1.45m²
Ice coagulating capacity	4kg	9kg	12kg	18kg
Shelf temperature	70℃~+80℃			-65℃~+70℃
Cold trap temperature	-70/-90℃	-70/-90℃	-70℃/-90℃	-70℃/-85℃
Cold trap volume	9L	15L	18L	30L
chamber type	single	double
Shelf size	275*400 mm	275*400 mm	365*465 mm	365*465 mm
Shelf spacing	100mm(adjustable)	100mm(adjustable	70mm(adjustable)	70mm(adjustable)
Sample quantity (4R Cillin bottle)	558	1395	2630	4230
Shelf heating and cooling method	Thermal conducting silicone oil medium(temperature resistance： -100℃~+600℃)
Chamber and shelf surface	High vacuum stainless steel tube/KF25-40
vacuum line	High vacuum stainless steel tube			KF50
External valve port	6/	12/	18/	一
equipment power	3.8kw	5.5kw	6.5kw	8kw
equipment size	700*588*1600 mm	1030*700*1630 mm	1030*700*1850 mm	1500*850*1900 mm
Capping mode	Hydraulic system gland device/no gland			hydraulic pressure
Suitable clean room	Suitable
Vacuum sensor type	Pirani Vacuum sensor/Capacitive vacuum sensor
Remote control and alarm	Support 2G/4G/GSM/Wi-Fi
Terminal judgment function	Pressure contrast method	Pressure test/pressure contrast method
Freeze dryer front door cover	Stainless steel/Plexiglass			Stainless steel
Inert gas filling	(Optional) Sufficient amount and time for the program to run itself
User function customization	Can be customized and modified according to user needs
sterilization method	H2O2/steam sterilization