DBCO-NHCO-PEG5-acid

Everything you need to run a trial PACE Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

About

Everything you need to run a trial PACE® allele-specific PCR Genotyping Reaction on your existing lab equipment. Each PACE Trial Kit includes Test DNA samples, PACE Genotyping Assays, PACE Master Mix and a comprehensive PACE Genotyping Trial Kit Manual.

WHO IS THIS TRIAL KIT FOR?

Anyone who wants to try PACE genotyping reagents in their lab for the first time with a set of validated DNA samples, SNP assays and PACE Master Mix.

TRIAL KIT OVERVIEW

Step 1. Dispense each of the three trial DNA samples (DNA 1, 2 and 3) plus water (No Template Control) in triplicate onto a PCR plate using the suggested volumes.

Step 2. Combine appropriate volumes of PACE Genotyping Master Mix with PACE Genotyping Assay in a tube, as directed, then mix.

Step 3. Dispense the combined mixtures into each of the wells containing DNA using volumes indicated. Each test now contains a complete PACE Genotyping Reaction.

Step 4. Seal your PCR plate with an optically clear seal and centrifuge to ensure all components are at the bottom of the wells.

Step 5.Thermally cycle the reaction plate using the thermal cycling conditions provided.

Step 6. Read the plate and compare data produced with the expected results provided in the manual. Simple!

PACE MECHANISM

More information on the PACE genotyping chemistry and how it works can be found here: www.3crbio.com/#pace. PACE allele-specific PCR is used for the detection of SNPs, Indels and other sequence variants.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• PCR-grade water

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

PCR-grade water

• Set of three Solid Phase Adsorption Toxin Tracking (SPATT) Bags
• Pre activated
• Ready for deployment
• HP20

Description

Solid Phase Adsorption Toxin Tracking (SPATT) is a biomimetic in-situ water monitoring tool that falls under an expanding umbrella of passive samplers. It serves to warn researchers of toxin-producing harmful algal bloom (HAB) developments early on. It has been popularized through its affordability, ease of use, and its ability to capture ephemeral events in marine, brackish, and freshwater environments. Its uptake of contaminants has been shown to be more similar than other sampling methods to that of aquatic species like bivalves, mussels, and clams. It provides an average bioavailable fraction of a toxin over deployment time that can be used to determine an overall toxin risk to organisms. The sampling period typically depends on the bioactivity at a site, ranging from 24 hours to 4 weeks in most cases.

A SPATT passively absorbs and desorbs extracellular compounds over its stretch of time at a sampling site; in an organism, a toxin would go through biochemical detoxification processes. Passive samplers have a higher sensitivity for more compounds and provide improved stability and preservation of these compounds within the resin. SPATT devices capture less commonly detected cyanotoxins (e.g. cylindrospermopsin) at lower concentrations than that of a grab sample (collected at one point in time). Grab samples are limited in scope and sensitivity, and underrepresent toxins like microcystin-LR, which is picked up very reliably through SPATT technology.

Uses HP20 that is widely applicable for many toxins.

Used to capture:

• Cyanotoxin (e.g. microcystin and cylindrospermopsin)
• Saxitoxin & derivatives (GNTXs, C-toxins), and other paralytic shellfish toxins (PSTs)
• Domoic acid (DA)
• Nodularin
• Anatoxin-a
• Brevetoxins (via “red tide”-causing dinoflagellate Karenia brevis)
• Okadaic acid (OA) & derivative Dinophysistoxins (DTXs) and other Diarrheic shellfish poisoning (DSP) toxins
• Yessotoxin (YTX) and Pectenotoxins (PTXs)
• Cyclic imines (CIs), e.g. Spirolides (SPXs), Gymnodimines (GYMs), Pinnatoxins (PnTXs)
• Ciguatoxin (CTX) & precursor Gambiertoxin (GTX), Maitotoxin (MTx), and other ciguatera fish poisoning (CFP) toxins

Set of three Solid Phase Adsorption Toxin Tracking (SPATT) Bags
Pre activated
Ready for deployment
HP20

Description

Specifications

Clone	IHC697
Source	Mouse Monoclonal
Positive Control	Colon Cancer
Dilution Range	1:200

Thymidylate Synthase (TS) is a crucial enzyme responsible for the synthesis of 2′-deoxythymidine-5′-monophosphate (dTMP) a precursor for thymidylate which is necessary for DNA replication and repair from 2′-deoxyuridine-5′-monophosphate (dUMP). In terms of cancer, TS is an important target for cancer treatment as the inhibition of TS and therefore nucleotide synthesis necessary for cell growth has shown to be a vital part for successful treatment against colorectal, pancreatic and breast cancers.