Propargyl-PEG4-(CH2)3-methyl ester consists of a propargyl group and a methyl ester. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions to form a stable triazole linkage. Under strong basic condition, methyl ester can be hydrolyzed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG4-(CH2)3-methyl ester consists of a propargyl group and a methyl ester. The propargyl group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions to form a stable triazole linkage. Under strong basic condition, methyl ester can be hydrolyzed. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-YBGL

SKU: 700004358

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	β-Glucan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	510
Signal Response:	Increase
Linear Range:	4 to 100 μg of glucose per assay
Limit of Detection:	1 g/100 g
Total Assay Time:	~ 100 min
Application examples:	Yeast preparations, mushroom preparations and other materials.
Method recognition:	Novel method

The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.

We are happy to announce a new and improved procedure!

Trehalase enzyme has now been added to the β-Glucan Assay Kit (Yeast and Mushroom), further increasing the accuracy of β-glucan measurement for certain samples. The addition of trehalase removes instances of overestimation of β-glucan content in samples containing trehalose. Please refer to the assay protocol and the FAQ here for more information.

This product replaces the Enzymatic Yeast β-Glucan Assay Kit (K-EBHLG) as the method of measurement for β-glucan measurement in yeast samples. See here for more information. 

See our complete list of available polysaccharide assay kits.

Advantages

• Very cost effective
• All reagents stable for > 12 months after preparation 
• Only enzymatic kit available 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.

Introduction

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 100-150mg stool sample
Applications	RT-PCR, Northern hybridization and other experiments
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Stool
Sample amount	100-150 mg
Elution volume	≥30μl
Time per run	≤50 minutes
Liquid carrying volume per column	100µg
Binding yield of column	800µl

Principle

The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.

Advantages

• High purity – unique adsorbent for more efficient removal of inhibitors
• High concentration – maximum extraction of total RNA from stool samples
• Sensitive – RNA can be purified at the level of PG

Kit Contents

Contents	R418502	R418503
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
Glass Beads (0.1~0.6mm)	30 g	150 g
Buffer SPL	30 ml	140 ml
Buffer PHC	30 ml	140 ml
Buffer GRP	60 ml	250 ml
Buffer RW1	50 ml	250 ml
Buffer RW2 *	20 ml	2 x 50 ml
RNase Free Water	15 ml	30 ml

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.