DBCO-NHCO-PEG5-NHS ester is a PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-NHCO-PEG5-NHS ester is a PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
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Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)
The kits were developed for plasmid miniprep directly from bacterial cultures. 100% centrifuge-free; NO vacuum needed; No column needed.
Plasmid MiniPrep Kit (Magnetic Beads), Cat.# 50012 – Use 2 ml of bacteria culture directly
Plasmid MiniPrep High Throughput Kit (Magnetic Beads), Cat.# 50011 – High throughput: Use 0.2 ml of bacteria culture directly with 96-well plates – Low throughput: Use 0.2 ml of bacteria culture directly with 1.5 ml tubes
The kits can be used for both high copy and low copy numbers of plasmid DNA. With BioDynami’s proprietary magnetic beads technology, the kits eliminate the requirement of centrifuge, vacuum, and column. Our magnetic beads provide a robust and reliable tool for both high throughput and low throughput applications of plasmid DNA isolation with high binding capacity and fast magnetic response time.
The High Through kit (Cat.# 50011) can be used for both high throughput and low throughput extraction of plasmid DNA. Up to 96 samples can be extracted simultaneously when using a 96-well plate.
Yield of plasmid DNA may vary dependent on the bacteria strain, plasmid type, copy numbers, and growth conditions etc. DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, molecular cloning, restriction enzymatic digestion, transfection, and transformation etc. The isolated plasmid DNA with the magnetic beads is free of contaminations such as RNA, bacterial DNA, proteins, and other impurities.
Plasmid DNA were loaded on 1% of agarose gel after extraction.
Comparison of workflows of Magnetic Beads based kits
Features
100% centrifuge-free
Culture medium can be used directly without centrifuge to pellet the bacteria
Simple
No centrifuge
No column
No vacuum
Flexible applications with simplified miniprep protocol
High throughput: 96-well plates with 0.2 ml of each culture (Cat.# 50011)
Low throughput: 1.5 ml tubes with 0.2 ml of each culture (Cat.# 50011)
Low throughput: 1.5 ml tubes with 2 ml of each culture (Cat.# 50012)
Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt (25 count)
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Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
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Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.
1. Mini-7 centrifuge is widely used in laboratory and hospital.
2. This model can fit in 2ml, 1.5ml, 0.5ml and 0.2ml tubes. 3. Light weight, small size and beautiful design. 4. Easily control, super speed protection.
Mini-7 Technical Data
Max speed
7000rpm
Volume
6×0.2ml
6×0.5ml
6×1.5ml
6x2ml
8x2x0.5ml
Speed accuracy
≦±1%
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mini centrifuge also called palm centrifuge, we can supply mini centrifuge from 4000rpm to 12000rpm, also we have mini centrifuge with digital display.
